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Titolo:
HIGH EXPRESSION OF NAKED PLASMID DNA IN MUSCLES OF YOUNG RODENTS
Autore:
DANKO I; WILLIAMS P; HERWEIJER H; ZHANG G; LATENDRESSE JS; BOCK I; WOLFF JA;
Indirizzi:
UNIV WISCONSIN,WAISMAN CTR,DEPT PEDIAT MADISON WI 53705 UNIV WISCONSIN,WAISMAN CTR,DEPT PEDIAT MADISON WI 53705
Titolo Testata:
Human molecular genetics
fascicolo: 9, volume: 6, anno: 1997,
pagine: 1435 - 1443
SICI:
0964-6906(1997)6:9<1435:HEONPD>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
DIRECT GENE-TRANSFER; DUCHENNE MUSCULAR-DYSTROPHY; MOUSE SKELETAL-MUSCLE; GENETICALLY MODIFIED MYOBLASTS; CREATINE-KINASE GENE; SYSTEMIC DELIVERY; IN-VIVO; TRANSCRIPTIONAL REGULATION; INTRAMUSCULAR INJECTION; FACTOR-IX;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
51
Recensione:
Indirizzi per estratti:
Citazione:
I. Danko et al., "HIGH EXPRESSION OF NAKED PLASMID DNA IN MUSCLES OF YOUNG RODENTS", Human molecular genetics, 6(9), 1997, pp. 1435-1443

Abstract

There is a time window at 2 weeks of age for achieving very high levels of foreign gene expression from the intramuscular injection of naked plasmid DMA in mice and rats, The highest expression, over 1 mu g ofluciferase protein/muscle, was obtained in Balb/C mice using constructs containing the CMV promoter, a chimeric intron and the luc(+) luciferase gene, Approximately 50% of the myofibers were intensely blue following the intramuscular injection of a beta-galactosidase expression vector in 2 week old Balb/C mice, The effects of age, mouse strain andconstruct were multiplicative, resulting in >1000-fold greater luciferase and similar to 20-fold more beta-galactosidase-positive cells. These high levels of expression were unstable and were not observed in larger animals (dog, rhesus monkey), These results indicate that enormous levels of foreign gene expression can be obtained in muscle with naked DNA in vivo and will enable the temporary effects of gene functionand expression in rodent muscle to be expeditiously studied.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 13:57:10