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Titolo:
EXCELLENCE OF THE 2-LAYER METHOD (UNIVERSITY-OF-WISCONSIN SOLUTION PERFLUOROCHEMICAL) IN PANCREAS PRESERVATION BEFORE ISLET ISOLATION
Autore:
TANIOKA Y; SUTHERLAND DER; KURODA Y; GILMORE TR; ASAHEIM TC; KRONSON JW; LEONE JP;
Indirizzi:
UNIV MINNESOTA,SCH MED,DEPT SURG,BOX 280 UMHC,420 DELAWARE ST SE MINNEAPOLIS MN 55455 UNIV MINNESOTA,SCH MED,DEPT SURG MINNEAPOLIS MN 55455 KOBE UNIV,SCH MED,DEPT SURG 1 KOBE HYOGO 650 JAPAN
Titolo Testata:
Surgery
fascicolo: 2, volume: 122, anno: 1997,
pagine: 435 - 441
SICI:
0039-6060(1997)122:2<435:EOT2M(>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLD-STORAGE METHOD; RAT PANCREAS; CANINE PANCREAS; COLLAGENASE DIGESTION; UW SOLUTION; OXYGENATION; TEMPERATURE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
Y. Tanioka et al., "EXCELLENCE OF THE 2-LAYER METHOD (UNIVERSITY-OF-WISCONSIN SOLUTION PERFLUOROCHEMICAL) IN PANCREAS PRESERVATION BEFORE ISLET ISOLATION", Surgery, 122(2), 1997, pp. 435-441

Abstract

Background. In islet transplantation pancreatic preservation before islet isolation is an obstacle compromising islet yield and viability. We tested the feasibility of a two-layer method (University of Wisconsin solution. [UW]/perfluorochemical) for pancreatic preservation before islet isolation. Methods. Dog pancreases were processed into pure islets by the method of Ricordi preceded by five different preservations(groups 1-a and 1-b, the two-layer method for 3 and 24 hours; groups 2-a and 2-b, simple cold storage in UW for 3 and 24 hours; group 3, without preservation). Islet yields and functional success after autotransplantation into the liver were compared among the groups. Results. Postpurification islet equivalents (IE)/gm pancreas and functional success rate were 5600 (mean) 83% in group 1-a; 4000, 56% in group 1-b; 4700, 33% in group 2-a; 1300, 0% in group 2-b; and 5000, 89% in group 3 (p < 0.05; 26 versus 1-a, 1-b, and 3), respectively. There was no statistical difference among groups 1-a, 1-b, and 3 in terms of islet yield and function (p > 0.2). Conclusions. The two-layer method is more effective than conventional simple cold storage in UW for pancreatic preservation before islet isolation. Clinical trials with the two-layer method are warranted.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 08:58:29