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Titolo:
INSULIN STIMULATES THE RELEASE OF THE GLYCOSYL PHOSPHATIDYLINOSITOL-ANCHORED MEMBRANE DIPEPTIDASE FROM 3T3-L1 ADIPOCYTES THROUGH THE ACTIONOF A PHOSPHOLIPASE-C
Autore:
MOVAHEDI S; HOOPER NM;
Indirizzi:
UNIV LEEDS,DEPT BIOCHEM & MOL BIOL LEEDS LS2 9JT W YORKSHIRE ENGLAND UNIV LEEDS,DEPT BIOCHEM & MOL BIOL LEEDS LS2 9JT W YORKSHIRE ENGLAND
Titolo Testata:
Biochemical journal
, volume: 326, anno: 1997,
parte:, 2
pagine: 531 - 537
SICI:
0264-6021(1997)326:<531:ISTROT>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
KIDNEY MICROVILLAR MEMBRANE; RENAL DIPEPTIDASE; ECTO-ENZYMES; PLASMA-MEMBRANE; CELL-SURFACE; PIG BRAIN; PROTEINS; PURIFICATION; AMINOPEPTIDASE; LIVER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
51
Recensione:
Indirizzi per estratti:
Citazione:
S. Movahedi e N.M. Hooper, "INSULIN STIMULATES THE RELEASE OF THE GLYCOSYL PHOSPHATIDYLINOSITOL-ANCHORED MEMBRANE DIPEPTIDASE FROM 3T3-L1 ADIPOCYTES THROUGH THE ACTIONOF A PHOSPHOLIPASE-C", Biochemical journal, 326, 1997, pp. 531-537

Abstract

Membrane dipeptidase (MDP; EC 3.4.13.19) enzymic activity that was inhibited by cilastatin has been detected on the surface of 3T3-L1 cells. On differentiation of the cells from fibroblasts to adipocytes the activity of MDP increased 12-fold. Immunoelectrophoretic blot analysis indicated that on adipogenesis the increase in the amount of MDP preceded the appearance of GLUT-4. MDP on 3T3-L1 adipocytes was anchored inthe bilayer by a glycosyl phosphatidylinositol (GPI) moiety as evidenced by its release into the medium in a hydrophilic form on treatment of the cells with bacterial phosphatidylinositol-specific phospholipase C and the appearance of the inositol 1,2-cyclic monophosphate cross-reacting determinant. Incubation of 3T3-L1 adipocytes with either insulin or the sulphonylurea glimepiride led to a rapid concentration-and time-dependent release of MDP from the cell surface. The hydrophilic form of MDP released from the cells on stimulation with insulin was recognized by antibodies against the inositol 1,2-cyclic monophosphate cross-reacting determinant, indicating that it had been generated by cleavage of its GPI anchor through the action of a phospholipase C.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 00:43:33