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Titolo:
EXPANSION IN-VITRO OF TRANSPLANTABLE HUMAN CORD-BLOOD STEM-CELLS DEMONSTRATED USING A QUANTITATIVE ASSAY OF THEIR LYMPHO-MYELOID REPOPULATING ACTIVITY IN NONOBESE DIABETIC-SCID SCID MICE/
Autore:
CONNEALLY E; CASHMAN J; PETZER A; EAVES C;
Indirizzi:
BRITISH COLUMBIA CANC RES CTR,TERRY FOX LAB,601 W 10TH AVE VANCOUVER BC V5Z 1L3 CANADA UNIV BRITISH COLUMBIA,DEPT PATHOL & LAB MED VANCOUVER BC V5Z 1L3 CANADA UNIV BRITISH COLUMBIA,DEPT MED GENET VANCOUVER BC V5Z 1L3 CANADA
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 18, volume: 94, anno: 1997,
pagine: 9836 - 9841
SICI:
0027-8424(1997)94:18<9836:EIOTHC>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN HEMATOPOIETIC-CELLS; BONE-MARROW CELLS; COLONY-STIMULATING FACTOR; PROGENITOR CELLS; IN-VIVO; PERIPHERAL-BLOOD; LIMITING-DILUTION; FUNCTIONAL-CHARACTERIZATION; COMPETITIVE REPOPULATION; RECONSTITUTING ABILITY;
Keywords:
HEMATOPOIETIC STEM CELLS; TRANSPLANTATION; IN VITRO CELL EXPANSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
48
Recensione:
Indirizzi per estratti:
Citazione:
E. Conneally et al., "EXPANSION IN-VITRO OF TRANSPLANTABLE HUMAN CORD-BLOOD STEM-CELLS DEMONSTRATED USING A QUANTITATIVE ASSAY OF THEIR LYMPHO-MYELOID REPOPULATING ACTIVITY IN NONOBESE DIABETIC-SCID SCID MICE/", Proceedings of the National Academy of Sciences of the United Statesof America, 94(18), 1997, pp. 9836-9841

Abstract

Human hematopoiesis originates in a population of stem cells with transplantable lympho-myeloid reconstituting potential, but a method for quantitating such cells has not been available, We now describe a simple assay that meets this need, It is based on the ability of sublethally irradiated immunodeficient nonobese diabetic-scid/scid (NOD/SCID) mice to be engrafted by intravenously injected human hematopoietic cells and uses limiting dilution analysis to measure the frequency of human cells that produce both CD34-CD19(+) (B-lymphoid) and CD34(+) (myeloid) colony-forming cell progeny in the marrow of such recipients 6 to 8 weeks post-transplant. Human cord blood (CB) contains approximate to5 of these competitive repopulating units (CRU) per mi that have a similar distribution between the CD38(-) and CD38(+) subsets of CD34(+) CB cells as long-term culture-initiating cells (LTC-IC) (4:1 vs, 2:1),Incubation of purified CD34(+)CD38(-) human CB cells in serum-free medium containing fit-3 ligand, Steel factor, interleukin 3, interleukin6, and granulocyte colony-stimulating factor for 5-8 days resulted ina 100-fold expansion of colony-forming cells, a 4-fold expansion of LTC-IC, and a 2-fold (but significant, P < 0.02) increase in CRU, The culture-derived CRU, like the original CB CRU, generated pluripotent, erythroid, granulopoietic, megakaryopoietic, and pre-B cell progeny upon transplantation into NOD/SCID mice, These findings demonstrate an equivalent phenotypic heterogeneity amongst human CB cells detectable asCRU and LTC-IC, In addition, their similarly modest response to stimulation by a combination of cytokines that extensively amplify LTC-IC from normal adult marrow underscores the importance of ontogeny-dependent changes in human hematopoietic stem cell proliferation and self-renewal.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/20 alle ore 07:52:45