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Titolo:
REARRANGEMENT OF THE TUBULIN AND ACTIN CYTOSKELETON DURING PROGRAMMEDCELL-DEATH IN DROSOPHILA SALIVARY-GLANDS
Autore:
JOCHOVA J; ZAKERI Z; LOCKSHIN RA;
Indirizzi:
QUEENS UNIV,DEPT BIOL KINGSTON ON K7L 3N6 CANADA CUNY,QUEENS COLL,DEPT BIOL FLUSHING NY 11371 CUNY,GRAD CTR FLUSHING NY 11371 ST JOHNS UNIV,DEPT BIOL SCI JAMAICA NY 11432
Titolo Testata:
Cell death and differentiation
fascicolo: 2, volume: 4, anno: 1997,
pagine: 140 - 149
SICI:
1350-9047(1997)4:2<140:ROTTAA>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
ICE-LIKE PROTEASES; ULTRASTRUCTURAL ANALYSIS; DNA FRAGMENTATION; INDUCED APOPTOSIS; ACID-PHOSPHATASE; HL-60 CELLS; MELANOGASTER; MICROTUBULES; LARVAL; ACTIVATION;
Keywords:
MICROTUBULES; ACTIN FILAMENTS; PROGRAMMED CELL DEATH; DROSOPHILA SALIVARY GLANDS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
J. Jochova et al., "REARRANGEMENT OF THE TUBULIN AND ACTIN CYTOSKELETON DURING PROGRAMMEDCELL-DEATH IN DROSOPHILA SALIVARY-GLANDS", Cell death and differentiation, 4(2), 1997, pp. 140-149

Abstract

During larva-to-pupa metamorphosis Drosophila salivary glands undergoprogrammed cell death by autophagocytosis. Although ultrastructure ofDrosophila salivary glands has been extensively studied in the past, little is known about mechanism of programmed cell death, especially the role of the cytoskeleton. In this paper we describe changes in microtubule and actin filament network compared to the progress of DNA fragmentation and redistribution of acid phosphatase. In feeding and wandering larvae microtubules and actin filaments form regular networks localized mostly along the plasma membrane. The first major rearrangement of microtubules and actin filaments occurred when larvae everted spiracles and the glands shifted their secretion from saliva to mucoprotein glue (stage L1), Microtubule cytoskeleton became denser and actin filaments concentrated along eel boundaries. At the same time nuclei flattened and migrated into the microtubule-rich layer near the basal membrane. In late prepupae (8-10 h after P1) the microtubule network became fainter, and actin filaments appeared frequently deeper in cytoplasm, gradually concentrating around nuclei. Simultaneously large patches of acid phosphatase activity surrounded nuclei and shortly thereafter chromosomal DNA began to fragment. During the final collapse of the gland (early pupae, 13.5 h after formation of white puparium) cellularfragments and autophagic vacuoles contained a continuous F-actin lining and the microtubule network displayed signs of extensive degradation. The results are consistent with the hypothesis that, in Drosophila salivary glands, extensive autophagic activities target nuclei for degradation; that this process occurs late in the course of programmed cell death; and that it directly involves cytoskeletal structures which are altered far earlier during the course of cell death.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 12:07:21