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Titolo:
REGULATED POLY(A) TAIL SHORTENING IN SOMATIC-CELLS MEDIATED BY CAP-PROXIMAL TRANSLATIONAL REPRESSOR PROTEINS AND RIBOSOME ASSOCIATION
Autore:
MUCKENTHALER M; GUNKEL N; STRIPECKE R; HENTZE MW;
Indirizzi:
EUROPEAN MOL BIOL LAB,GENE EXPRESS PROGRAMME,MEYERHOFSTR 1 D-69117 HEIDELBERG GERMANY EUROPEAN MOL BIOL LAB,GENE EXPRESS PROGRAMME D-69117 HEIDELBERG GERMANY
Titolo Testata:
RNA
fascicolo: 9, volume: 3, anno: 1997,
pagine: 983 - 995
SICI:
1355-8382(1997)3:9<983:RPTSIS>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA TRANSLATION; IRON-RESPONSIVE-ELEMENT; XENOPUS OOCYTE MATURATION; HUMAN FERRITIN EXPRESSION; 5' UNTRANSLATED REGION; CYTOPLASMIC POLYADENYLATION; OXIDATIVE STRESS; GENE-EXPRESSION; MEIOTIC MATURATION; BINDING PROTEIN;
Keywords:
IRON-RESPONSIVE ELEMENT; MESSENGER-RNA; POLY(A) TAIL; RNA PROCESSING; RNA/PROTEIN INTERACTION; TRANSLATIONAL CONTROL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
71
Recensione:
Indirizzi per estratti:
Citazione:
M. Muckenthaler et al., "REGULATED POLY(A) TAIL SHORTENING IN SOMATIC-CELLS MEDIATED BY CAP-PROXIMAL TRANSLATIONAL REPRESSOR PROTEINS AND RIBOSOME ASSOCIATION", RNA, 3(9), 1997, pp. 983-995

Abstract

The poly(A) tail plays an important role in translation initiation. We report the identification of a mechanism that operates in mammalian somatic cells, and couples mRNA poly(A) tail length with its translation state. The regulation of human ferritin L-chain mRNA by iron-responsive elements (IRPs) and iron regulatory proteins (IRPs) is subject tothis mechanism: translational repression imposed by IRP binding to the IRE of ferritin L-chain mRNA induces poly(A) tail shortening. For the accumulation of mRNAs with short poly(A) tails, IRP binding to an IRE per se is not sufficient, but must cause translational repression. Interestingly, puromycin and verrucarin (general translation inhibitorsthat dissociate mRNAs from ribosomes) mimick the negative effect of the specific translational repressor proteins on poly(A) tail length, whereas cycloheximide and anisomycin (general translation inhibitors that maintain the association between mRNAs and ribosomes) preserve longpoly(A) tails. Thus, the ribosome association of the mRNA appears to represent the critical determinant. These findings identify a novel mechanism of regulated polyadenylation as a consequence of translationalcontrol. They reveal differences in poly(A) tail metabolism between polysomal and mRNP-associated mRNAs. A possible role of this mechanism in the maintenance of translational repression is discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 09:53:11