Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
DECODING THE TRANSLATIONAL TERMINATION SIGNAL - THE POLYPEPTIDE-CHAINRELEASE FACTOR IN ESCHERICHIA-COLI CROSS-LINKS TO THE BASE FOLLOWING THE STOP CODON
Autore:
POOLE ES; BRIMACOMBE R; TATE WP;
Indirizzi:
UNIV OTAGO,DEPT BIOCHEM,POB 56 DUNEDIN NEW ZEALAND UNIV OTAGO,DEPT BIOCHEM DUNEDIN NEW ZEALAND UNIV OTAGO,CTR GENE RES DUNEDIN NEW ZEALAND
Titolo Testata:
RNA
fascicolo: 9, volume: 3, anno: 1997,
pagine: 974 - 982
SICI:
1355-8382(1997)3:9<974:DTTTS->2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
SUPPRESSOR TRANSFER-RNA; PEPTIDYL-TRANSFER-RNA; ELONGATION-FACTOR-G; CRYSTAL-STRUCTURE; NASCENT PEPTIDE; UGA SUPPRESSION; TERNARY COMPLEX; MESSENGER-RNA; GENETIC-CODE; FACTOR-II;
Keywords:
MOLECULAR MIMICRY; RELEASE FACTOR-II; SITE-DIRECTED CROSS-LINKING; TERMINATION COMPLEX;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
E.S. Poole et al., "DECODING THE TRANSLATIONAL TERMINATION SIGNAL - THE POLYPEPTIDE-CHAINRELEASE FACTOR IN ESCHERICHIA-COLI CROSS-LINKS TO THE BASE FOLLOWING THE STOP CODON", RNA, 3(9), 1997, pp. 974-982

Abstract

Protein release factors act like tRNA analogues in decoding translational stop signals. Statistical analysis of the sequences at translational stop sites and functional studies with particular signals indicatethis mimicry involves an increase in the length of the signal in the mRNA. The base following the stop codon (+4 base) is of particular interest because it has a strong influence on the competitiveness of the stop signal at recoding sites, suggesting it might form part of the release factor recognition element. Site-directed crosslinking from the 4 base showed that it is in close proximity to the Escherichia coli release factor-2 in a termination complex, a prerequisite for the +4 base being part of the recognition element. Fingerprinting analysis indicates that crosslinking to the release factor occurred from both +1 and +4 positions of the stop signal in the same RNA molecule. This provides more evidence that the +4 base may be an integral part of the decoding signature in the mRNA during the termination phase of protein biosynthesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 09:36:11