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Titolo:
IDENTIFICATION OF A MICROTUBULE-ASSOCIATED MOTOR PROTEIN ESSENTIAL FOR DENDRITIC DIFFERENTIATION
Autore:
SHARP DJ; YU WQ; FERHAT L; KURIYAMA R; RUEGER DC; BAAS PW;
Indirizzi:
UNIV WISCONSIN,DEPT ANAT,SCH MED,1300 UNIV AVE MADISON WI 53706 UNIV WISCONSIN,DEPT ANAT,SCH MED MADISON WI 53706 UNIV WISCONSIN,DEPT ANAT,PROGRAM NEUROSCI MADISON WI 53706 UNIV MINNESOTA,SCH MED,DEPT CELL BIOL & NEUROANAT MINNEAPOLIS MN 55455 CREAT BIOMOL HOPKINTON MA 01748
Titolo Testata:
The Journal of cell biology
fascicolo: 4, volume: 138, anno: 1997,
pagine: 833 - 843
SICI:
0021-9525(1997)138:4<833:IOAMMP>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLARITY ORIENTATION; HIPPOCAMPAL-NEURONS; GROWING AXONS; CYTOPLASMIC DYNEIN; SPINDLE FORMATION; SF9 CELLS; TRANSPORT; MITOSIS; ORGANIZATION; CULTURE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
D.J. Sharp et al., "IDENTIFICATION OF A MICROTUBULE-ASSOCIATED MOTOR PROTEIN ESSENTIAL FOR DENDRITIC DIFFERENTIATION", The Journal of cell biology, 138(4), 1997, pp. 833-843

Abstract

The quintessential feature of the dendritic microtubule array is its nonuniform pattern of polarity orientation. During the development of the dendrite, a population of plus end-distal microtubules first appears, and these microtubules are subsequently joined by a population of oppositely oriented microtubules. Studies from our laboratory indicatethat the latter microtubules are intercalated within the microtubule array by their specific transport from the cell body of the neuron during a critical stage in development (Sharp, D.J., W. Yu, and P.W. Baas. 1995. J. Cell Biol. 130:93-104). In addition, we have established that the mitotic motor protein termed CHO1/MKLP1 has the appropriate properties to transport microtubules in this manner (Sharp, D.J., R. Kuriyama, and P.W. Baas. 1996. J. Neurosci. 16:4370-4375). In the present study we have sought to determine whether CHO1/MKLP1 continues to be expressed in terminally postmitotic neurons and whether it is required for the establishment of the dendritic microtubule array. In situ hybridization analyses reveal that CHO1/MKLP1 is expressed in postmitotic cultured rat sympathetic and hippocampal neurons. Immunofluorescence analyses indicate that the motor is absent from axons but is enriched in developing dendrites, where it appears as discrete patches associated with the microtubule array. Treatment of the neurons with antisense oligonucleotides to CHO1/MKLP1 suppresses dendritic differentiation, presumably by inhibiting the establishment of their nonuniform microtubule polarity pattern. We conclude that CHO1/MKLP1 transports microtubules from the cell body into the developing dendrite with their minus ends leading, thereby establishing the nonuniform microtubule polarity pattern of the dendrite.

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Documento generato il 05/12/20 alle ore 14:02:42