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Titolo:
FOLLICLE-STIMULATING-HORMONE AND INTRACELLULAR 2ND-MESSENGER REGULATESTEROIDOGENIC ACUTE REGULATORY PROTEIN MESSENGER-RIBONUCLEIC-ACID IN LUTEINIZED PORCINE GRANULOSA-CELLS
Autore:
PESCADOR N; HOUDE A; STOCCO DM; MURPHY BD;
Indirizzi:
UNIV MONTREAL,FAC MED VET,CTR RECH REPROD ANIM,3200 RUE SICOTTE ST HYACINTHE PQ J2S 7C6 CANADA UNIV MONTREAL,FAC MED VET,CTR RECH REPROD ANIM ST HYACINTHE PQ J2S 7C6 CANADA TEXAS TECH UNIV,HLTH SCI CTR,DEPT BIOCHEM & CELL BIOL LUBBOCK TX 79430
Titolo Testata:
Biology of reproduction
fascicolo: 3, volume: 57, anno: 1997,
pagine: 660 - 668
SICI:
0006-3363(1997)57:3<660:FAI2R>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYCLOHEXIMIDE-INSENSITIVE MECHANISM; HUMAN CHORIONIC-GONADOTROPIN; LEYDIG TUMOR-CELLS; KINASE-C; GENE-EXPRESSION; ANGIOTENSIN-II; ADRENAL-CELLS; RAT OVARY; STAR GENE; DEHYDROGENASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
N. Pescador et al., "FOLLICLE-STIMULATING-HORMONE AND INTRACELLULAR 2ND-MESSENGER REGULATESTEROIDOGENIC ACUTE REGULATORY PROTEIN MESSENGER-RIBONUCLEIC-ACID IN LUTEINIZED PORCINE GRANULOSA-CELLS", Biology of reproduction, 57(3), 1997, pp. 660-668

Abstract

Ligand-and second messenger-regulated expression of the gene for steroidogenic acute regulatory protein (StAR) was evaluated in luteinized porcine granulosa cells. For comparison, cytochrome P450 side-chain cleavage (P450(scc)) was examined. Northern hybridization with homologous cDNA probes demonstrated three StAR mRNA species, of 2.7, 1.6, and 0.8 kilobases (kb), with the smallest variably present, and a single P450(scc) band at 1.9 kb. FSH elevated both StAR and P450(scc) messages in a dose-dependent manner over 6 h and continually stimulated both over 24 h (p < 0.001). StAR message induction depended on transcription,as did that of P450(scc). Over 6 h, actinomycin D eliminated constitutive StAR message and reduced that of P450(scc) by two thirds, indicating briefer persistence of StAR. Pretreatment with cycloheximide prevented FSH induction of StAR and P450(scc) mRNA, implicating intermediate protein synthesis in expression of both genes. Dibutyryl cAMP causedtime-dependent increases in StAR and P450 mRNAs over 24 h (p < 0.001), indicating the importance of the protein kinase A (PKA) pathway in their gene expression. Activation of the protein kinase C (PKC) pathwayby a phorbol ester eliminated FSH induction of StAR mRNA increases (p< 0.01) while only reducing P450(scc) induction (p < 0.05). Thus, StAR gene expression, as reflected in mRNA abundance, is regulated by FSHvia the PKA pathway and is dependent on transcription and translation. Conversely, the PKC pathway inhibits induction of these important steroid synthetic genes in luteinized granulosa cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 22:16:54