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Titolo:
EXPRESSION OR AROMATASE CYTOCHROME-P450 PROTEIN AND MESSENGER-RIBONUCLEIC-ACID IN HUMAN ENDOMETRIOTIC AND ADENOMYOTIC TISSUES BUT NOT IN NORMAL ENDOMETRIUM
Autore:
KITAWAKI J; NOGUCHI T; AMATSU T; MAEDA K; TSUKAMOTO K; YAMAMOTO T; FUSHIKI S; OSAWA Y; HONJO H;
Indirizzi:
KYOTO PREFECTURAL UNIV MED,DEPT OBSTET & GYNECOL,KAMIKYO KU,KAWARAMACHI HIROKOJI KYOTO 602 JAPAN KYOTO PREFECTURAL UNIV MED,DEPT DYNAM PATHOL,RES INST NEUROL DIS & GERIATR KYOTO 602 JAPAN HAUPTMAN WOODWARD MED RES INST INC,ENDOCRINE BIOCHEM DEPT BUFFALO NY 14203
Titolo Testata:
Biology of reproduction
fascicolo: 3, volume: 57, anno: 1997,
pagine: 514 - 519
SICI:
0006-3363(1997)57:3<514:EOACPA>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHAIN-REACTION AMPLIFICATION; LINKED-IMMUNOSORBENT-ASSAY; HUMAN UTERINE LEIOMYOMA; IMMUNOHISTOCHEMICAL LOCALIZATION; PROGESTERONE RECEPTORS; STROMAL CELLS; NEOPLASTIC ENDOMETRIUM; MONOCLONAL-ANTIBODY; ESTROGEN SYNTHESIS; MENSTRUAL-CYCLE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
J. Kitawaki et al., "EXPRESSION OR AROMATASE CYTOCHROME-P450 PROTEIN AND MESSENGER-RIBONUCLEIC-ACID IN HUMAN ENDOMETRIOTIC AND ADENOMYOTIC TISSUES BUT NOT IN NORMAL ENDOMETRIUM", Biology of reproduction, 57(3), 1997, pp. 514-519

Abstract

To determine whether local estrogen production lakes place in endometriotic or adenomyotic tissues, in eutopic endometrium from patients with endometriosis or adenomyosis, and in normal endometrium, tissue specimens were examined try immunohistochemistry, catalytic activity, andmRNA expression for aromatase cytochrome P450 (P450(arom)). P450(arom) was immunohistochemically localized in the cytoplasm of glandular cells of endometriotic and adenomyotic tissues, and of eutopic endometrium from patients with the respective diseases, whereas estrogen receptors and progesterone receptors were localized in the nuclei of the glandular cells and stroma. Aromatase activity in the microsomal fractionof adenomyotic tissues was inhibited by the addition of danazol, aromatase inhibitors, and anti-human placental P450(arom) monoclonal antibody (mAb3-2C2) in a manner similar to such inhibition in other human tissues. Reverse transcription polymerase chain reaction and Southern blot analysis also revealed P450(arom) mRNA in these tissues. However, neither P450(arom) protein activity nor mRNA was detected in endometrial specimens obtained from normal menstruating women with cervical carcinoma in situ but without any other gynecological disease. These results suggest that at a local level, endometriotic and adenomyotic tissues produce estrogens, which may be involved in the tissue growth through interacting with the estrogen receptor.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 18:44:46