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Titolo:
GLOBULAR AND FIBROUS STRUCTURE IN BARLEY CHROMOSOMES REVEALED BY HIGH-RESOLUTION SCANNING ELECTRON-MICROSCOPY
Autore:
IWANO M; FUKUI K; TAKAICHI S; ISOGAI A;
Indirizzi:
NARA INST SCI & TECHNOL,GRAD SCH BIOL SCI NARA 63001 JAPAN HOKURIKU NATL AGR EXPTL STN,DEPT BREEDING JOETSU 94301 JAPAN NATL CARDIOVASC CTR,DEPT ETIOL & PATHOPHYSIOL OSAKA 565 JAPAN
Titolo Testata:
Chromosome research
fascicolo: 5, volume: 5, anno: 1997,
pagine: 341 - 349
SICI:
0967-3849(1997)5:5<341:GAFSIB>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-DEPLETED CHROMOSOMES; HELA METAPHASE CHROMOSOMES; PLANT CHROMOSOMES; CENTROMERIC REGION; CHROMATIN; ORGANIZATION; DNA; ULTRASTRUCTURE; SCAFFOLD; NUCLEAR;
Keywords:
BARLEY CHROMOSOME; HIGH-RESOLUTION SCANNING ELECTRON MICROSCOPY; SURFACE STRUCTURE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
52
Recensione:
Indirizzi per estratti:
Citazione:
M. Iwano et al., "GLOBULAR AND FIBROUS STRUCTURE IN BARLEY CHROMOSOMES REVEALED BY HIGH-RESOLUTION SCANNING ELECTRON-MICROSCOPY", Chromosome research, 5(5), 1997, pp. 341-349

Abstract

Barley chromosomes were prepared for high-resolution scanning electron microscopy using a combination of enzyme maceration, treatment in acetic acid and osmium impregnation using thiocarbohydrazide. Using thistechnique, the three-dimensional ultrastructure of interphase nuclei and mitotic chromosomes was examined. In interphase, different levels of chromatin condensation were observed, consisting of fibrils 10 nm in diameter, 20- to 40-nm fibres and a higher order complex. In prophase, globular and strand-like structures composed of 20- to 40-nm fibreswere dominant. As the cells progressed through the cell cycle and thechromatin condensed, globular and strand-like structures (chromomeres) were coiled and packed to form chromosomes. Chromomeres were observed as globular protuberances on the surface of metaphase chromosomes. These findings indicate that the chromomere is a fundamental substructure of the higher order architecture of the chromosome. In the centromeric region, there were no globular protuberances, but 20- to 40-nm fibres were folded compactly to form a higher level organization surrounding the chromosomal axis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 16:06:44