Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
TOPOGRAPHICAL STRUCTURE OF PBR322 DNA STUDIED BY SCANNING TUNNELING MICROSCOPE AND ATOMIC-FORCE MICROSCOPE
Autore:
ZHANG PC; BAI C; CHENG YJ; FANG Y; WANG ZH; HUANG XT;
Indirizzi:
CHINESE ACAD SCI,INST CHEM,STM LAB BEIJING 100080 PEOPLES R CHINA NANKAI UNIV,DEPT BIOL TIANJIN PEOPLES R CHINA
Titolo Testata:
Journal of vacuum science & technology. B, Microelectronics and nanometer structures processing, measurement and phenomena
fascicolo: 3, volume: 12, anno: 1994,
pagine: 1461 - 1464
SICI:
1071-1023(1994)12:3<1461:TSOPDS>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOLECULES; DYNAMICS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
10
Recensione:
Indirizzi per estratti:
Citazione:
P.C. Zhang et al., "TOPOGRAPHICAL STRUCTURE OF PBR322 DNA STUDIED BY SCANNING TUNNELING MICROSCOPE AND ATOMIC-FORCE MICROSCOPE", Journal of vacuum science & technology. B, Microelectronics and nanometer structures processing, measurement and phenomena, 12(3), 1994, pp. 1461-1464

Abstract

Plasmid pBR322 DNA (0.5 mg/ml) isolated from Eschericha coli HB101 was suspended in Tris-HC1/EDTA (1/0.1 mol/L, pH 8.5), deposited on a highly oriented pyrolytic graphite surface, then imaged with scanning tunneling microscope (STM) in air and under paraffin liquid in constant current mode. The methods for preparing DNA samples used in the STM study are discussed. Dynamic changes of apparent width of supercoiled pBR322 DNA were captured during an STM imaging course. The pBR322 DNA is also visualized with an atomic force microscope (AFM) built by this laboratory after the sample was absorbed on the freshly cleaved mica surface. Characteristically relaxed circular structures of pBR322 DNA have been observed in the AFM experiment.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 10:23:36