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Titolo:
RECOMBINANT HUMAN IMMUNODEFICIENCY PR55(GAG) VIRUS-LIKE PARTICLES PRESENTING CHIMERIC ENVELOPE GLYCOPROTEINS INDUCE CYTOTOXIC T-CELLS AND NEUTRALIZING ANTIBODIES
Autore:
DEML L; SCHIRMBECK R; REIMANN J; WOLF H; WAGNER R;
Indirizzi:
KLINIKUM REGENSBURG,INST MED MICROBIOL,FRANZ JOSEF STR ALLEE 11 D-93053 REGENSBURG GERMANY UNIV REGENSBURG,INST MED MICROBIOL D-93053 REGENSBURG GERMANY UNIV ULM,INST MED MICROBIOL & IMMUNOL D-89069 ULM GERMANY
Titolo Testata:
Virology
fascicolo: 1, volume: 235, anno: 1997,
pagine: 26 - 39
SICI:
0042-6822(1997)235:1<26:RHIPVP>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
TOXIC LYMPHOCYTES-T; BLOOD MONONUCLEAR-CELLS; CELLULAR IMMUNE-RESPONSES; SURFACE-ANTIGEN PARTICLES; PERIPHERAL-BLOOD; IMMUNODOMINANT EPITOPE; SYNTHETIC PEPTIDE; SEQUENCE IDENTITY; TYPE-1 INFECTION; FINE SPECIFICITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
75
Recensione:
Indirizzi per estratti:
Citazione:
L. Deml et al., "RECOMBINANT HUMAN IMMUNODEFICIENCY PR55(GAG) VIRUS-LIKE PARTICLES PRESENTING CHIMERIC ENVELOPE GLYCOPROTEINS INDUCE CYTOTOXIC T-CELLS AND NEUTRALIZING ANTIBODIES", Virology, 235(1), 1997, pp. 26-39

Abstract

Very recently, we demonstrated that the replacement of the human immunodeficiency virus type-1 (HIV-1) gp41 transmembrane protein by an Epstein-Barr virus gp220/350-derived membrane anchor resulted in the incorporation of chimeric envelope (Env) oligomers into pr55(gag) virus-like particles (VLPs), exceeding that of wild-type gp160 by a factor of 10, In this study, we examined the immunostimulatory properties of Pr55(gag) VLPs to both (i) chimeric HIV-1 gp120 external envelope proteins and (ii) full-length gp160 presented on the outer surface of the particles. immunization studies carried out with VLPs presenting different derivatives of the chimeric and wild-type Enu proteins elicited a consistent anti-Pr55(gag) as well as anti-Env antibody response in complete absence of additional adjuvants. In both cases, the immune sera exhibited an in vitro neutralizing activity against homologous HIV-1 infection in MT4 cells, Noteworthy, these VLPs were also capable of inducing a strong CD8+ cytotoxic T-cell (CTL) response in immunized BALB/c mice that was directed toward a known CTL epitope in the third variable domain V3 of the gp120 external glycoprotein. However, the inductionof V3-loop-specific CTLs critically depended on the amounts of Env proteins that were presented by the Pr55(gag) VLPs. Moreover, the CD8(+)CTL response was not significantly altered by adsorbing the VLPs to alum or by repeated boaster immunizations. These results illustrate that Pr55(gag) VLPS provide a safe and effective means of enhancing neutralizing humoral responses to particle-entrapped gp120 proteins and arealso capable of delivering these proteins to the MHC class i antigen processing and presentation pathway. Therefore, antigenically expandedPr55(gag) VLPS represent an attractive approach in the design of vaccines for which specific stimulation of neutralizing antibodies and cytotoxic effector functions to complex glycoproteins is desired. (C) 1997 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/08/20 alle ore 23:28:55