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Titolo:
TISSUE-SPECIFIC CYTOKINE PRODUCTION DURING EXPERIMENTAL ACUTE-PANCREATITIS - A PROBABLE MECHANISM FOR DISTANT ORGAN DYSFUNCTION
Autore:
NORMAN JG; FINK GW; DENHAM W; YANG J; CARTER G; SEXTON C; FALKNER J; GOWER WR; FRANZ MG;
Indirizzi:
UNIV S FLORIDA,DEPT SURG 112,13000 BRUCE B DOWNS BLVD TAMPA FL 33612 UNIV S FLORIDA,PANCREAS STUDY GRP TAMPA FL 33612
Titolo Testata:
Digestive diseases and sciences
fascicolo: 8, volume: 42, anno: 1997,
pagine: 1783 - 1788
SICI:
0163-2116(1997)42:8<1783:TCPDEA>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; FACTOR-ALPHA; LUNG INJURY; RAT; EXPRESSION; MEDIATORS; INDUCTION; SURVIVAL; SEVERITY; CELLS;
Keywords:
INTERLEUKIN-1; INTERLEUKIN-6; TUMOR NECROSIS FACTOR; GENE EXPRESSION; ACUTE PANCREATITIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
J.G. Norman et al., "TISSUE-SPECIFIC CYTOKINE PRODUCTION DURING EXPERIMENTAL ACUTE-PANCREATITIS - A PROBABLE MECHANISM FOR DISTANT ORGAN DYSFUNCTION", Digestive diseases and sciences, 42(8), 1997, pp. 1783-1788

Abstract

Our purpose was to determine if cytokines are produced systemically during acute pancreatitis. Proinflammatory cytokines are elevated during acute pancreatitis and have been implicated in the progression of pancreatitis-associated multiple organ dysfunction. Whether these mediators are produced within all tissues or very few specific organs is notknown. Edematous pancreatitis was induced in adult male mice by IP injection of cerulein. Necrotizing pancreatitis was induced in young female mice by feeding a choline-deficient, ethionine supplemented diet. Animals were sacrificed as pancreatitis worsened, with multiple organsprepared for tissue mRNA and protein analysis by RT-PCR and immunoblotting. Pancreatitis severity was established by histologic grading andserum amylase and lipase. There was no cytokine mRNA or protein detectable prior to the induction of pancreatitis. Tumor necrosis factor-alpha (TNF-alpha) and interleukin-l-beta (IL-1 beta) mRNA and protein were detected within the pancreas early in the course of pancreatitis inboth models, coinciding with the development of hyperamylasemia (bothP < 0.001). Interleukin-6 was produced in the pancreas after pancreatitis was more fully developed (P < 0.001). IL-1 beta and TNF-alpha were subsequently produced in large amounts in lung, liver, and spleen but never within kidney, cardiac muscle, or skeletal muscle. A significant delay between pancreatic and distant organ cytokine production was always observed. It is concluded that proinflammatory cytokines are produced within the pancreas and within organs known to develop dysfunction during severe pancreatitis. Cytokine production is tissue specific, correlates with disease severity, and occurs within the pancreas first and subsequently within distant organs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/10/20 alle ore 13:53:47