Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
EFFECTS OF CHANGES IN BLOOD-FLOW RATE ON CELL-DEATH AND CELL-PROLIFERATION IN CAROTID ARTERIES OF IMMATURE RABBITS
Autore:
CHO A; MITCHELL L; KOOPMANS D; LANGILLE BL;
Indirizzi:
TORONTO HOSP,RES INST,BANTING & BEST DIABET CTR,CCRW 1-836,200 ELIZABETH ST TORONTO ON M5G 2C4 CANADA TORONTO HOSP,RES INST,BANTING & BEST DIABET CTR TORONTO ON M5G 2C4 CANADA UNIV TORONTO,DEPT PATHOL TORONTO ON CANADA
Titolo Testata:
Circulation research
fascicolo: 3, volume: 81, anno: 1997,
pagine: 328 - 337
SICI:
0009-7330(1997)81:3<328:EOCIBR>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
INDUCED APOPTOSIS; ENDOTHELIAL-CELLS; PERINATAL-PERIOD; FETAL SHEEP; B-CELLS; EXPRESSION; BIRTH; BCL-2; HYPERPLASIA; REGRESSION;
Keywords:
BLOOD FLOW; CELL DEATH; APOPTOSIS; CELL PROLIFERATION; ARTERIAL REMODELING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
A. Cho et al., "EFFECTS OF CHANGES IN BLOOD-FLOW RATE ON CELL-DEATH AND CELL-PROLIFERATION IN CAROTID ARTERIES OF IMMATURE RABBITS", Circulation research, 81(3), 1997, pp. 328-337

Abstract

Spontaneous and experimental changes in arterial blood flow rates affect tissue accumulation in developing arteries. To examine whether cell proliferation and/or cell death are affected by alterations in bloodflow, we ligated the left external carotid artery of 3-week-old rabbits, which reduces left common carotid blood flow by 71%. In control arteries and after 2 days of flow reduction, agarose gel electrophoresisof DNA extracted from all carotid arteries resolved multiple low molecular weight bands characteristic of apoptosis; however, DNA fragmentation in arteries carrying reduced blood how was 2.5-fold higher than that of control arteries. The effect of reduced blood flow on cell death subsequently waned but remained significant at 7 days. Cell death incarotid arteries was also detected by in vivo uptake of propidium iodide, a DNA-binding fluorescent dye that labels the nuclei of nonviablecells. Both smooth muscle and endothelial cells exhibited large and statistically significant increases in labeling index in the flow-reduced artery. Propidium iodide-labeled cells were cleared from the vesselwall within 1 to 4 hours of labeling, and nuclear staining displayed condensation (clumping) of chromatin in all labeled cells at later time points. This time course and nuclear morphology and the rapid clearance of labeled cells are consistent with death via apoptosis. Many propidium iodide-positive cells did not display chromatin condensation immediately after labeling; however, this was also true of cultured endothelial cells that were driven into apoptosis with sphingomyelinase treatment and then double-labeled with propidium iodide and the apoptosis marker annexin V. We infer that propidium iodide can label apoptoticvascular cells before these cells display chromatin condensation thatis detectable with fluorescence labeling of DNA. Replication rates ofsmooth muscle and endothelial cells, determined by 5-bromo-2'-deoxyuridine uptake, were inhibited by >75% with decreased blood flow. The inhibition of proliferation was unabated after 7 days of reduced flow. These findings indicate that the coordinated regulation of cell death and cell proliferation, in response to changes in arterial blood flow rates, contributes to arterial remodeling during development.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 05:19:21