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Titolo:
EXPOSURE TO LOW-OXYGEN TENSION AND INCREASED OSMOLARITY ENHANCE THE ABILITY OF MYCOBACTERIUM-AVIUM TO ENTER INTESTINAL EPITHELIAL (HT-29) CELLS
Autore:
BERMUDEZ LE; PETROFSKY M; GOODMAN J;
Indirizzi:
CALIF PACIFIC MED CTR,RES INST,KUZELL INST ARTHRITIS & INFECT DIS,2200 WEBSTER ST,ROOM 305 SAN FRANCISCO CA 94115 UNIV CALIF SAN FRANCISCO,DEPT PEDIAT,ELECTRON MICROSCOPY LAB SAN FRANCISCO CA 94143
Titolo Testata:
Infection and immunity
fascicolo: 9, volume: 65, anno: 1997,
pagine: 3768 - 3773
SICI:
0019-9567(1997)65:9<3768:ETLTAI>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACQUIRED-IMMUNODEFICIENCY-SYNDROME; VIRULENCE DETERMINANTS; ESCHERICHIA-COLI; YERSINIA-ENTEROCOLITICA; BACTERIAL VIRULENCE; GENE-EXPRESSION; INV GENE; COMPLEX; SALMONELLA; INFECTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
L.E. Bermudez et al., "EXPOSURE TO LOW-OXYGEN TENSION AND INCREASED OSMOLARITY ENHANCE THE ABILITY OF MYCOBACTERIUM-AVIUM TO ENTER INTESTINAL EPITHELIAL (HT-29) CELLS", Infection and immunity, 65(9), 1997, pp. 3768-3773

Abstract

Current evidence indicates that Mycobacterium avium infection in patients with AIDS is acquired mostly through the gastrointestinal (GI) tract and that M. avium binds to and invades GI mucosal cells in vitro, Since M. avium is exposed to specific environmental conditions in the GI tract such as changes in pH, low oxygen (O-2) tension, increased osmolarity, and low concentration of iron, we investigated the effects of these conditions on the bacterium's ability to enter HT-29 intestinal cells, M. avium 101 (serovar 1) was cultured in 7H9 broth and then exposed to pH 4.5 to 8.0, low O-2 tension, 0.1 to 0.3 M dextrose, and absence of iron for 2 h, After washing, bacteria (10(7)/ml) were used in the invasion assay, Confluent HT-29 cells were exposed to 10(6) bacteria for 1 h and then treated with amikacin (200 mu g/ml) for 2 h to selectively kill extracellular but not intracellular M. avium. The supernatant was then removed, the monolayer was lysed, and the lysate was plated onto 7H10 agar plates, While exposure to acidic and basic pHs, as well as iron free medium, had no significant effect on M. avium invasion of intestinal epithelial cells, low O-2 tension and increased osmolarity enhanced invasion 11- and 9-fold, respectively, compared withthe control, Exposure of M. avium to the combination of low O-2 concentration and hyperosmolarity resulted in an approximate 10- to 11-foldincrease in penetration of HT-29 cells. Hyperosmolarity and low O-2 tension induced the invasive M. avium phenotype and can be useful for the identification of genes associated with M. avium invasion of intestinal mucosa.

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Documento generato il 24/10/20 alle ore 11:30:17