Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
5-CARBOXYFLUORESCEIN DIACETATE AS A PROBE FOR MEASURING THE GROWTH OFKERATINOCYTES
Autore:
HANTHAMRONGWIT M; REID WH; COURTNEY JM; GRANT MH;
Indirizzi:
UNIV STRATHCLYDE,BIOENGN UNIT GLASGOW G4 0NW SCOTLAND UNIV STRATHCLYDE,BIOENGN UNIT GLASGOW G4 0NW SCOTLAND GLASGOW ROYAL INFIRM,BURNS UNIT GLASGOW G4 0NW SCOTLAND
Titolo Testata:
Human & experimental toxicology
fascicolo: 6, volume: 13, anno: 1994,
pagine: 423 - 427
SICI:
0960-3271(1994)13:6<423:5DAAPF>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
DIFFERENTIATION; PROLIFERATION; COLLAGEN; CULTURE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
14
Recensione:
Indirizzi per estratti:
Citazione:
M. Hanthamrongwit et al., "5-CARBOXYFLUORESCEIN DIACETATE AS A PROBE FOR MEASURING THE GROWTH OFKERATINOCYTES", Human & experimental toxicology, 13(6), 1994, pp. 423-427

Abstract

There is a requirement for a convenient and reliable method for evaluating the growth rate of human keratinocytes cultured on collagen-based substrates. Therefore, three methods of determining cell growth werefirst used to quantify the growth rate of the well-characterised L929mouse fibroblast cell line on tissue culture plastic and the results compared. The methods used were the measurement of total cell protein,cell counting using an electronic Coulter counter and a fluorimetric assay employing 5-carboxyfluorescein diacetate (CFDA). The CFDA assay showed the highest correlation with seeding density of the L929 cells,and the lowest standard deviations. It was the most rapid and convenient method for processing large numbers of samples. Only viable cells can deacetylate the non-fluorescent CFDA to carboxyfluorescein, which is fluorescent and accumulates inside the cells. Therefore, the assay specifically quantifies only viable cells. Subsequently, this assay has been successfully applied to the measurement of human keratinocyte growth rate on collagen gels and sponges. We have demonstrated that keratinocytes grow equally well on gels and sponges, and that media containing low calcium concentrations (0.09 mM) favour rapid proliferation of keratinocytes. Our results show that the CFDA assay is an accurate,reliable and convenient method for quantifying cell growth in vitro. It is particularly valuable when growing cells on optically opaque substrata, such as collagen sponges, where growth cannot be monitored daily by microscopy.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 15:16:37