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Titolo:
INDUCTION OF APOPTOSIS IN MV1LU CELLS BY EXPRESSION OF COMPETITIVE RB1 MUTANTS
Autore:
WHITAKER LL; HANSEN MF;
Indirizzi:
TEMPLE UNIV,SCH MED,FELS INST CANC RES & MOL BIOL,3307 N BROAD ST PHILADELPHIA PA 19140 UNIV TEXAS,MD ANDERSON CANCER CTR,DEPT MOL GENET HOUSTON TX 77030
Titolo Testata:
Oncogene
fascicolo: 9, volume: 15, anno: 1997,
pagine: 1069 - 1077
SICI:
0950-9232(1997)15:9<1069:IOAIMC>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
RETINOBLASTOMA GENE-PRODUCT; E2F TRANSCRIPTION FACTOR; HUMAN LYMPHOID NEOPLASMS; LARGE T-ANTIGEN; SUSCEPTIBILITY GENE; HUMAN CANCER; FUNCTIONAL INTERACTIONS; PROTEIN; BINDING; MUTATIONS;
Keywords:
DOMINANT-NEGATIVE; GROWTH SUPPRESSION DOMAIN; TUMOR SUPPRESSOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
79
Recensione:
Indirizzi per estratti:
Citazione:
L.L. Whitaker e M.F. Hansen, "INDUCTION OF APOPTOSIS IN MV1LU CELLS BY EXPRESSION OF COMPETITIVE RB1 MUTANTS", Oncogene, 15(9), 1997, pp. 1069-1077

Abstract

The Retinoblastoma tumor suppressor gene (RB1) plays a role in a variety of human cancers, Experimental analyses have indicated that the protein product of the RB1 gene (pRb) plays a role in cell cycle regulation. pRb function is dependent on its ability to bind to cellular factors, which is facilitated by multiple protein binding domains within pRb. Mutations within these domains which eliminate the ability of pRb to bind its targets result in loss of function. Although loss of pRb function may lead to uncontrolled cellular proliferation, tumorigenesisis not the only response to pRb inactivation. Examination of various tissues from RB1-nullizygous mouse embryos showed problems in differentiation and induction of apoptosis, suggesting that the ultimate response to the loss of pRb is influenced by cellular context. It has previously been demonstrated in RB1-negative Saos-2 cells that co-expression of the C-terminal domain of pRb with wildtype pRb resulted in escapefrom G1 arrest and continued cell cycling. In this paper we demonstrate that in RB1-positive Mv1Lu cells, expression of the A/B pocket region or the N-terminus (when combined with a nuclear localization signal), but not the C-terminal domain, is able to competitively disrupt thefunction of the endogenous pRb, and that the result of this disruption is apoptosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 09:40:32