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Titolo:
A HUMAN COLONIC CELL-LINE SHARING SIMILARITIES WITH ENTEROCYTES AS A MODEL TO EXAMINE ORAL ABSORPTION - ADVANTAGES AND LIMITATIONS OF THE CACO-2 MODEL
Autore:
DELIE F; RUBAS W;
Indirizzi:
GENENTECH INC,IDNA WAY S SAN FRANCISCO CA 94080 GENENTECH INC S SAN FRANCISCO CA 94080
Titolo Testata:
Critical reviews in therapeutic drug carrier systems
fascicolo: 3, volume: 14, anno: 1997,
pagine: 221 - 286
SICI:
0743-4863(1997)14:3<221:AHCCSS>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTESTINAL EPITHELIAL-CELLS; BORDER MEMBRANE-VESICLES; PROTEIN-KINASE-C; THYROTROPIN-RELEASING-HORMONE; CARRIER-MEDIATED TRANSPORT; EPIDERMAL GROWTH-FACTOR; SERUM-FREE MEDIUM; DIPEPTIDE GLYCYLSARCOSINE TRANSPORT; DRUG PERMEABILITY COEFFICIENTS; SUCRASE-ISOMALTASE EXPRESSION;
Keywords:
CACO-2 CULTURE; ORAL ABSORPTION; PERMEABILITY; DRUG SCREENING; IN VITRO;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
281
Recensione:
Indirizzi per estratti:
Citazione:
F. Delie e W. Rubas, "A HUMAN COLONIC CELL-LINE SHARING SIMILARITIES WITH ENTEROCYTES AS A MODEL TO EXAMINE ORAL ABSORPTION - ADVANTAGES AND LIMITATIONS OF THE CACO-2 MODEL", CR R THER, 14(3), 1997, pp. 221-286

Abstract

Caco-2 cell monolayers mimic intestinal absorptive epithelium and represent a very useful tool for studying transepithelial transport. The literature on Caco-2 cells is controversial regarding transepithelial resistance and permeabilities of different marker compounds across monolayers. This paper discusses probable causes for these discrepancies. First, we present the role of culture conditions, such as the nature of the support or the passage number, on cell biology and transport properties. Further, we compare the presence of transport proteins in Caco-2 cells to mammalian intestinal tissue and discuss their implication for drug absorption. We also examine the advantages and disadvantages of systems such as Transwell(R) and side-by-side diffusion chambers. A summary of comparisons between permeabilities across Caco-2 monolayers and mammalian intestinal tissues is provided. We conclude that theorigin of Caco-2 cells and the culture conditions are in part responsible for the discrepancies encountered in the literature.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 18:09:32