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Titolo:
APPLICATION OF HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY TO THE STUDY OFTHE BIOLOGICAL TRANSFORMATION OF ADIPONITRILE
Autore:
MOREAU JL; AZZA S; BIGEY F; ARNAUD A; GALZY P;
Indirizzi:
ECOLE NATL SUPER AGRON MONTIPELLIER,CHAIR MICROBIOL IND & GENET MICOORGANISMES,PL VIALA F-34060 MONTPELLIER FRANCE ECOLE NATL SUPER AGRON MONTIPELLIER,CHAIR MICROBIOL IND & GENET MICOORGANISMES F-34060 MONTPELLIER FRANCE
Titolo Testata:
Journal of chromatography B. Biomedical applications
fascicolo: 1, volume: 656, anno: 1994,
pagine: 197 - 202
Fonte:
ISI
Lingua:
ENG
Soggetto:
WIDE SUBSTRATE SPECTRUM; BREVIBACTERIUM-SP; NITRILE-HYDRATASE; ACYLAMIDE AMIDOHYDROLASE; MUTANT STRAIN; PURIFICATION; AMIDASE; ACID;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
16
Recensione:
Indirizzi per estratti:
Citazione:
J.L. Moreau et al., "APPLICATION OF HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY TO THE STUDY OFTHE BIOLOGICAL TRANSFORMATION OF ADIPONITRILE", Journal of chromatography B. Biomedical applications, 656(1), 1994, pp. 197-202

Abstract

A procedure for the assay of nitrile hydratase and amidase activity by high-performance liquid chromatography is described. The method can be used to assay the intermediate compounds resulting from the hydrolysis of adiponitrile into adipic acid, and to determine the kinetics ofthe hydrolysis of these compounds using whole cells and enzyme extracts. The precision of the method makes it suitable for the determination of the enzyme parameters: K-m and V-m (nitrile hydratase and amidase). Using cyanovaleramide as substrate, K-m and V-m were respectively 370 mM and 2060 U/mg for nitrile hydratase and 6.6 mM and 33 U/mg for amidase.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 03:29:31