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Titolo:
GLYCINE-INDUCED LONG-TERM POTENTIATION IS ASSOCIATED WITH STRUCTURAL AND FUNCTIONAL MODIFICATIONS OF PHA-AMINO-3-HYDROXYL-5-METHYL-4-ISOXAZOLEPROPIONIC ACID RECEPTORS
Autore:
MUSLEH W; BI XN; TOCCO G; YAGHOUBI S; BAUDRY M;
Indirizzi:
UNIV SO CALIF,PROGRAM NEUROSCI,HEDCO NEUROSCI BLDG 309 LOS ANGELES CA90089 UNIV SO CALIF,PROGRAM NEUROSCI LOS ANGELES CA 90089
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 17, volume: 94, anno: 1997,
pagine: 9451 - 9456
SICI:
0027-8424(1997)94:17<9451:GLPIAW>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
HIPPOCAMPAL SLICES; SYNAPTIC RESPONSES; CALPAIN INHIBITORS; SILENT SYNAPSES; AMPA RECEPTORS; RAT-BRAIN; LTP; MEMORY; PROTEOLYSIS; PLASTICITY;
Keywords:
HIPPOCAMPUS; PLASTICITY; CALPAIN; GLUTAMATE; SPECTRIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
W. Musleh et al., "GLYCINE-INDUCED LONG-TERM POTENTIATION IS ASSOCIATED WITH STRUCTURAL AND FUNCTIONAL MODIFICATIONS OF PHA-AMINO-3-HYDROXYL-5-METHYL-4-ISOXAZOLEPROPIONIC ACID RECEPTORS", Proceedings of the National Academy of Sciences of the United Statesof America, 94(17), 1997, pp. 9451-9456

Abstract

Global long-term potentiation (LTP) was induced in organotypic hippocampal slice cultures by a brief application of 10 mM glycine, Glycine-induced LTP was occluded by previous theta burst stimulation-induced potentiation, indicating that both phenomena share similar cellular processes. Glycine-induced LTP was associated with increased [H-3] pha-amino-3-hydroxyl-5-methyl-4-isoxazolepropionic acid (AMPA) binding in membrane fractions as well as increased amount of a selective spectrin breakdown product generated by calpain-mediated spectrin proteolysis, Antibodies against the C-terminal (C-Ab) and N-terminal (N-Ab) domains of GluR1 subunits were used to evaluate structural changes in AMPA receptor properties resulting from glycine-induced LTP, No quantitative or qualitative changes were observed in Western blots from membrane fractions prepared from glycine-treated slices with C-Ab, In contrast, Western blots stained with N-Ab revealed the formation of a 98-kDa species of GluR1 subunits as well as an increased amount of immunoreactivity after glycine-induced LTP, The amount of spectrin breakdown product was positively correlated with the amount of the 98-kDa species of GluR1 after glycine treatment, Functional modifications of AMPA receptorswere evaluated by determining changes in the effect of pressure-applied AMPA on synaptic responses before and after glycine-induced LTP. Glycine treatment produced a significant increase in AMPA receptor function after potentiation that correlated with the degree of potentiation, The results indicate that LTP induction produces calpain activation,truncation of the C-Ab domain of GluR1 subunits of AMPA receptors, and increased AMPA receptor function. They also suggest that insertion of new receptors takes place after LTP induction.

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Documento generato il 20/01/21 alle ore 11:52:12