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Titolo:
CHROMATIN ASSEMBLY IN A YEAST WHOLE-CELL EXTRACT
Autore:
SCHULTZ MC; HOCKMAN DJ; HARKNESS TAA; GARINTHER WI; ALTHEIM BA;
Indirizzi:
UNIV ALBERTA,DEPT BIOCHEM EDMONTON AB T6G 2H7 CANADA
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 17, volume: 94, anno: 1997,
pagine: 9034 - 9039
SICI:
0027-8424(1997)94:17<9034:CAIAYW>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEWLY REPLICATED CHROMATIN; DNA-REPLICATION; FREE SYSTEM; XENOPUS OOCYTES; GENETIC-CONTROL; DIVISION CYCLE; IN-VITRO; INVITRO; TRANSCRIPTION; COMPLEXES;
Keywords:
NUCLEOSOME ASSEMBLY; HISTONE; SACCHAROMYCES CEREVISIAE; DNA REPAIR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
50
Recensione:
Indirizzi per estratti:
Citazione:
M.C. Schultz et al., "CHROMATIN ASSEMBLY IN A YEAST WHOLE-CELL EXTRACT", Proceedings of the National Academy of Sciences of the United Statesof America, 94(17), 1997, pp. 9034-9039

Abstract

A simple in vitro system that supports chromatin assembly was developed for Saccharomyces cerevisiae. The assembly reaction is ATP-dependent, uses soluble histones and assembly factors, and generates physiologically spaced nucleosomes, We analyze the pathway of histone recruitment into nucleosomes, using this system in combination with genetic methods for the manipulation of yeast, This analysis supports the model of sequential recruitment of H3/H4 tetramers and H2A/H2B dimers into nucleosomes, Using a similar approach, we show that DNA ligase I can play an important role in template repair during assembly, These studies demonstrate the utility of this system for the combined biochemical and genetic analysis of chromatin assembly in yeast.

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Documento generato il 25/11/20 alle ore 07:35:12