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Titolo:
MONITORING SERUM CONCENTRATIONS OF CLOMIPRAMINE AND METABOLITES - FLUORESCENCE POLARIZATION IMMUNOASSAY VERSUS HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
Autore:
BANGER M; HERMES B; HARTTER S; HIEMKE C;
Indirizzi:
UNIV ESSEN GESAMTHSCH,KLIN ALLGEMEINE PSYCHIAT,DEPT PSYCHIAT,VIRCHOWSTR 174 D-45147 ESSEN GERMANY UNIV MAINZ,DEPT PSYCHIAT D-6500 MAINZ GERMANY
Titolo Testata:
Pharmacopsychiatry
fascicolo: 4, volume: 30, anno: 1997,
pagine: 128 - 132
SICI:
0176-3679(1997)30:4<128:MSCOCA>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN PLASMA; TRICYCLIC ANTIDEPRESSANTS; HYDROXYLATED METABOLITES; DRUG; NORTRIPTYLINE; DISORDERS; TRENDS; HPLC;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
M. Banger et al., "MONITORING SERUM CONCENTRATIONS OF CLOMIPRAMINE AND METABOLITES - FLUORESCENCE POLARIZATION IMMUNOASSAY VERSUS HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY", Pharmacopsychiatry, 30(4), 1997, pp. 128-132

Abstract

This study compared fluorescence polarization immunoassay (FPIA) witha high performance liquid chromatographic (HPLC) method from the point of view of their applicability to therapeutic drug monitoring of patients treated with clomipramine alone. Blood was withdrawn from 20 depressed inpatients (54+/-14 years) under steady state conditions. The FPIA determined total tricyclic antidepressant (TCA) concentrations with day to day variability below 11%. The automated HPLC method separated clomipramine, N-desmethylclomipramine, 8-hydroxyclomipramine and 8-hydroxydesmethylclomipramine with interassay coefficients of variance below 12%. The concetrations measured by FPIA were similar to HPLC results. Total TCA concentrations measured by FPIA and the sum of clomipramine and desmethylclomipramine measured by HPLC correlated significantly (r=0.780 and p<0.01). However, 40% of individual FPIA determinations yielded results that differed by more than 50% from the HPLC concentrations. Changes in clinical rates were related only to TCA serum concentrations that had been analyzed by HPLC. It is concluded that the semiquantitative FPIA is unsuitable for therapeutic drug monitoring in patients under clomipramine treatment, whereas the differential analysis of clomipramine and metabolites by HPLC is informative and can be used to improve the antidepressant drug treatment.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 22:17:53