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Titolo:
GENETIC DIVERSITY AMONG A COMPLEX OF CEREAL CYST NEMATODES INFERRED FROM RFLP ANALYSIS OF THE RIBOSOMAL INTERNAL TRANSCRIBED SPACER REGION
Autore:
BEKAL S; GAUTHIER JP; RIVOAL R;
Indirizzi:
INST NATL RECH AGRON,CTR RECH RENNES,ZOOL LAB,BP 29 F-35653 LE RHEU FRANCE INST NATL RECH AGRON,CTR RECH RENNES,ZOOL LAB F-35653 LE RHEU FRANCE
Titolo Testata:
Genome
fascicolo: 4, volume: 40, anno: 1997,
pagine: 479 - 486
SICI:
0831-2796(1997)40:4<479:GDAACO>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
FRAGMENT-LENGTH-POLYMORPHISM; HETERODERA-AVENAE; REPETITIVE DNA; GLOBODERA; PATTERNS; KEY;
Keywords:
CEREAL CYST NEMATODES; HETERODERA AVENAE; PCR; RFLP; RIBOSOMAL DIVERSITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
S. Bekal et al., "GENETIC DIVERSITY AMONG A COMPLEX OF CEREAL CYST NEMATODES INFERRED FROM RFLP ANALYSIS OF THE RIBOSOMAL INTERNAL TRANSCRIBED SPACER REGION", Genome, 40(4), 1997, pp. 479-486

Abstract

This study examined the restriction polymorphism (RFLP) of the nuclear ribosomal DNA in Heterodera avenae, H. filipjevi, H. mani, H. latipons, and the taxonomically unclear Gotland strain in order to establisha molecular characterization and phylogenetic relationships in the complex of cereal cyst nematodes (CCN). The internal transcribed spacer (ITS) and 5.8S rDNA were amplified by PCR from a single female or a cyst of 27 different geographic isolates of the CCN complex and one population of H. schachtii, used as outgroup. The amplified product was 1.2 kb long and 14 of 15 enzymes produced restriction fragments for eachisolate. Relationships between populations were determined from UPGMAanalysis based on distance values calculated from RFLP data. Digestions with TaqI clearly differentiated H. avenae, H. latipons, and a group composed of H.filipjevi and the Gotland strain. Six endonucleases (HaeIII, HinfI, ItaI, PstI, TaqI, and Tru9I) produced the same restriction pattern with H. filipjevi and the Gotland strain, and both were clearly separated from H. avenae with PstI. Restriction sites have revealed a mixture of the species H. latipons and H. avenae, and possible infraspecific variation in H. avenae. The inferred phylogenetic relationships of species in the CCN complex are in agreement with their morphological characterization.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 14:14:06