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Titolo:
IDENTIFICATION OF THE PROTEIN-KINASE-C ISOENZYMES IN HUMAN LUNG AND AIRWAYS SMOOTH-MUSCLE AT THE PROTEIN AND MESSENGER-RNA LEVEL
Autore:
WEBB BLJ; LINDSAY MA; SEYBOLD J; BRAND NJ; YACOUB MH; HADDAD EB; BARNES PJ; ADCOCK IM; GIEMBYCZ MA;
Indirizzi:
NATL HEART & LUNG INST,UNIV LONDON IMPERIAL COLL SCI TECHNOL & MED,SCH MED,DOVEHOUSE ST LONDON SW3 6LY ENGLAND NATL HEART & LUNG INST,UNIV LONDON IMPERIAL COLL SCI TECHNOL & MED,SCH MED LONDON SW3 6LY ENGLAND
Titolo Testata:
Biochemical pharmacology
fascicolo: 1, volume: 54, anno: 1997,
pagine: 199 - 205
SICI:
0006-2952(1997)54:1<199:IOTPII>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
SIGNAL-TRANSDUCTION; PHORBOL ESTERS; LIGHT CHAIN; PHOSPHORYLATION; CELLS; CONTRACTION; EXPRESSION; MYOSIN; DIFFERENTIATION; DIACYLGLYCEROL;
Keywords:
HUMAN LUNG; HUMAN TRACHEAL SMOOTH MUSCLE; HUMAN TRACHEALIS; PROTEIN KINASE C; PKC MESSENGER-RNA; PKC MULTIPLICITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
B.L.J. Webb et al., "IDENTIFICATION OF THE PROTEIN-KINASE-C ISOENZYMES IN HUMAN LUNG AND AIRWAYS SMOOTH-MUSCLE AT THE PROTEIN AND MESSENGER-RNA LEVEL", Biochemical pharmacology, 54(1), 1997, pp. 199-205

Abstract

The protein kinase C (PKC) isoenzymes expressed by human peripheral lung and tracheal smooth muscle resected from individuals undergoing heart-lung transplantation were identified at the protein and mRNA level. Western immunoblot analyses of human lung identified multiple PKC isoenzymes that were differentially distributed between the soluble and particulate fraction. Thus, PKC alpha, PKC beta(II), PKC epsilon, and PKC zeta were recovered predominantly in the soluble fraction whereas the eta isoform was membrane-associated together with trace amounts ofPKC alpha and PKC epsilon. PKC beta(I)-like immunoreactivity was occasionally seen although the intensity of the band was uniformly weak. Immunoreactive bands corresponding to PKCs gamma, delta, or a were never detected. Reverse transcription-polymerase chain reaction (RT-PCR) of RNA extracted from human lung using oligonucleotide primer pairs that recognise unique sequences in each of the PKC genes amplified cDNA fragments that corresponded to the predicted sizes of PKC alpha, PKC beta(I), PKC beta(II), PKC epsilon, PKC zeta, and PKC eta (consistent with the expression of PKC isoenzyme protein) and, in addition, mRNA forPKC delta; PCR fragments of the expected size for the supposedly muscle-specific isoform, PKC theta, or the atypical isoenzyme, PKC lambda,were never obtained. The complement and distribution of PKC isoforms in human trachealis were similar, but not identical, to human lung. Thus, immunoreactive bands corresponding to the alpha, beta(I), beta(II), epsilon, and zeta isoenzymes of PKC were routinely labelled in the cytosolic fraction. In the particulate material PKC alpha, PKC epsilon,PKC alpha, PKC eta, and PKC mu were detected by immunoblotting. With the exception of PKC zeta, RT-PCR analyses confirmed the expression ofthe PKC isoforms detected at the protein level and, in addition, identified mRNA for PKC delta. Collectively, these data clearly demonstrate the expression of multiple PKC isoenzymes in human lung and trachealsmooth muscle, suggesting that they subserve diverse multifunctional roles in these tissues. (C) 1997 Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 01:13:09