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Titolo:
THYROID HORMONE-REGULATED EXPRESSION OF RC3 NEUROGRANIN IN THE IMMORTALIZED HYPOTHALAMIC CELL-LINE GT1-7/
Autore:
MORTE B; INIGUEZ MA; LORENZO PI; BERNAL J;
Indirizzi:
CSIC,INST INVEST BIOMED,ARTURO DUPERIER 4 E-28029 MADRID SPAIN CSIC,INST INVEST BIOMED E-28029 MADRID SPAIN
Titolo Testata:
Journal of neurochemistry
fascicolo: 3, volume: 69, anno: 1997,
pagine: 902 - 909
SICI:
0022-3042(1997)69:3<902:THEORN>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
KINASE-C SUBSTRATE; RC3 NEUROGRANIN; MESSENGER-RNA; PROTEIN; RECEPTOR; GENE; SEQUENCES; DIFFERENTIATION; IDENTIFICATION; NEURONS;
Keywords:
TRIIODOTHYRONINE; RECEPTORS; BRAIN; GENE EXPRESSION; HYPOTHYROIDISM; CRETINISM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
B. Morte et al., "THYROID HORMONE-REGULATED EXPRESSION OF RC3 NEUROGRANIN IN THE IMMORTALIZED HYPOTHALAMIC CELL-LINE GT1-7/", Journal of neurochemistry, 69(3), 1997, pp. 902-909

Abstract

The calmodulin-binding, protein kinase C substrate RCS/neurogranin isthe product of a neuron-specific gene expressed in the forebrain thatis under specific regional and temporal control by thyroid hormone (3,5,3'-triiodothyronine, T-3) In vivo, some neuronal populations are sensitive and others are insensitive to T-3. The goal of this study was to identify neuronal cell cultures that express RC3/neurogranin, to check whether they are sensitive to T-3, and to examine the mechanism ofregulation, We found that RC3 is induced by T-3 in the hypothalamic cell line GT1-7 at the transcriptional level. The half-life of the mature mRNA was 20 h and was not affected by the hormone. Addition of T-3 to the cell culture induces neurogranin mRNA after 6 h in the absence of new protein synthesis. These results suggest: a direct transcriptional effect of T-3 mediated through nuclear receptors. Indeed, GT1-7 cells express functional T-3 receptors, as shown by northern blotting, nuclear T-3-binding assays, and transactivation of reporter genes. The role of retinoic acid and glucocorticoids on RC3 expression was also evaluated, because we have previously noted the presence of consensus response elements for these hormones in the RC3 upstream promoter region. In contrast to T-3, neither retinoic acid nor dexamethasone influences neurogranin expression despite the presence of respective functional receptors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 13:18:20