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Titolo:
EXPRESSION OF A CHEMOTACTIC CYTOKINE (MCP-1) IN CEREBRAL CAPILLARY ENDOTHELIAL-CELLS IN-VITRO
Autore:
ZACH O; BAUER HC; RICHTER K; WEBERSINKE G; TONTSCH S; BAUER H;
Indirizzi:
AUSTRIAN ACAD SCI,INST MOL BIOL,BILLROTHSTR 11 A-5020 SALZBURG AUSTRIA SALZBURG UNIV,INST ZOOL A-5020 SALZBURG AUSTRIA SALZBURG UNIV,INST DEV BIOL & GENET A-5020 SALZBURG AUSTRIA
Titolo Testata:
Endothelium
fascicolo: 3, volume: 5, anno: 1997,
pagine: 143 - 153
SICI:
1062-3329(1997)5:3<143:EOACC(>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHEMOATTRACTANT PROTEIN-1 MCP-1; BLOOD MONONUCLEAR LEUKOCYTES; POLYMERASE CHAIN-REACTION; GROWTH-FACTOR; GENE-EXPRESSION; MESSENGER-RNA; SMOOTH-MUSCLE; CDNA CLONING; MONOCYTE; PURIFICATION;
Keywords:
ENDOTHELIAL CELLS; CHEMOKINES; CDNA CLONING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
O. Zach et al., "EXPRESSION OF A CHEMOTACTIC CYTOKINE (MCP-1) IN CEREBRAL CAPILLARY ENDOTHELIAL-CELLS IN-VITRO", Endothelium, 5(3), 1997, pp. 143-153

Abstract

A full-length cDNA encoding the porcine monocyte chemoattractant protein-1 (pMCPC-1) was isolated from growth-stimulated porcine cerebral capillary endothelial cells (cEC); the pMCP-1 cDNA showed 89% identity to human MCP-1 and was isolated by use of subtractive hybridization and differential screening of two phenotypically different subpopulations of cloned cEC. pMCP-1 was abundantly expressed in cEC grown in the presence of FCS, ECGF and heparin whereas lower expression was observedin cEC kept in FCS-supplemented medium only. As shown by Northern blot analysis, no pMCP-1 transcripts were present in total RNA derived from freshly isolated brain capillaries, large brain vessels or whole brain homogenate. MCP/JE expression was also demonstrated in ECGF/heparin-treated murine cEC. Astrocytes and smooth muscle cells grown in FCS-supplemented medium did not show MCP-1 expression. Treatment of porcine cEC with TNF-alpha increased pMCP-1 mRNA levels in a dose-dependent manner. These data further support the notion that cerebral capillary endothelial cells actively participate in processes of CNS host defense.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/10/20 alle ore 04:51:27