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Titolo:
MATRIX-ASSISTED LASER DESORPTION IONISATION MASS-SPECTROMETRY GUIDED PURIFICATION OF HUMAN GUANYLIN FROM BLOOD ULTRAFILTRATE/
Autore:
SCHRADER M; JURGENS M; HESS R; SCHULZKNAPPE P; RAIDA M; FORSSMANN WG;
Indirizzi:
LOWER SAXONY INST PEPTIDE RES,IPF,FEODOR LYNEN STR 31 D-30625 HANNOVER GERMANY
Titolo Testata:
Journal of chromatography
fascicolo: 1, volume: 776, anno: 1997,
pagine: 139 - 145
Fonte:
ISI
Lingua:
ENG
Soggetto:
FORM;
Keywords:
MATRIX-ASSISTED LASER DESORPTION/IONISATION MASS SPECTROMETRY; PEPTIDES; PROTEINS; HORMONES; GUANYLIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
11
Recensione:
Indirizzi per estratti:
Citazione:
M. Schrader et al., "MATRIX-ASSISTED LASER DESORPTION IONISATION MASS-SPECTROMETRY GUIDED PURIFICATION OF HUMAN GUANYLIN FROM BLOOD ULTRAFILTRATE/", Journal of chromatography, 776(1), 1997, pp. 139-145

Abstract

The purification of the human peptide hormone guanylin 22-115 from blood ultrafiltrate (hemofiltrate, HF) was achieved using matrix-assisted laser desorption/ionisation mass spectrometry (MALDI-MS) as the assay system. Screening a peptide bank generated from 5000 l HF guanylin 22-115 was detected by its molecular mass when adequate conditions for MALDI-MS analysis were chosen. The sensitivity was even better than ofthe established biological assay system. In addition, the susceptibility towards solvents and salts is strongly reduced. 1.2 mg of the peptide hormone was purified from 10% of the starting material. (C) 1997 Elsevier Science B.V.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 06:55:22