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Titolo:
CAPILLARY ELECTROPHORESIS IN THE ASSAY OF THE HYDROLYSIS OF GLYCINE-CONTAINING PEPTIDES BY A PROTEASE FROM PSEUDOMONAS-AERUGINOSA
Autore:
VESSILLIER S; BERNILLON J; SAULNIER J; WALLACH J;
Indirizzi:
UNIV LYON 1,UFR CHIM BIOCHIM,LAB BIOCHIM ANALYT & SYNTH BIOORGAN,BATIMENT 303 F-69622 VILLEURBANNE FRANCE UNIV LYON 1,UFR CHIM BIOCHIM,LAB BIOCHIM ANALYT & SYNTH BIOORGAN F-69622 VILLEURBANNE FRANCE
Titolo Testata:
Journal of chromatography
fascicolo: 1, volume: 776, anno: 1997,
pagine: 133 - 137
Fonte:
ISI
Lingua:
ENG
Soggetto:
SPECIFICITY; LASA; PURIFICATION;
Keywords:
PSEUDOMONAS AERUGINOSA; PEPTIDES; PROTEASES; PEPTIDES; PROTEASES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
12
Recensione:
Indirizzi per estratti:
Citazione:
S. Vessillier et al., "CAPILLARY ELECTROPHORESIS IN THE ASSAY OF THE HYDROLYSIS OF GLYCINE-CONTAINING PEPTIDES BY A PROTEASE FROM PSEUDOMONAS-AERUGINOSA", Journal of chromatography, 776(1), 1997, pp. 133-137

Abstract

A rapid and simple capillary zone electrophoresis (CZE) method for measuring the activity of a minor protease from Pseudomonas aeruginosa is described. When glycine-containing oligopeptides were used as substrates, it was possible to separate and quantify substrate and products. Moreover oligopeptide hydrolysates were analysed by fast atom bombardment mass spectrometry providing the sites of splitting of the substrates. By comparison with CZE calibration curves constructed with reference peptides, the initial rates of hydrolysis were calculated. The method, validated for pentaglycine hydrolysis, was also used for the analysis of reaction mixtures and for monitoring the enzymic hydrolysis ofvarious peptides in order to investigate enzyme specificity. (C) 1997Elsevier Science B.V.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 13:35:12