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Titolo:
A MITOCHONDRIAL-DNA PRIMASE FROM THE TRYPANOSOMATID CRITHIDIA-FASCICULATA
Autore:
LI CJ; ENGLUND PT;
Indirizzi:
JOHNS HOPKINS UNIV,SCH MED,DEPT BIOL CHEM BALTIMORE MD 21205 JOHNS HOPKINS UNIV,SCH MED,DEPT BIOL CHEM BALTIMORE MD 21205
Titolo Testata:
The Journal of biological chemistry
fascicolo: 33, volume: 272, anno: 1997,
pagine: 20787 - 20792
SICI:
0021-9258(1997)272:33<20787:AMPFTT>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
KINETOPLAST DNA; REPLICATION; MINICIRCLES; TOPOISOMERASE; LOCALIZATION; PURIFICATION; POLYMERASE; EVOLUTION; SITES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
C.J. Li e P.T. Englund, "A MITOCHONDRIAL-DNA PRIMASE FROM THE TRYPANOSOMATID CRITHIDIA-FASCICULATA", The Journal of biological chemistry, 272(33), 1997, pp. 20787-20792

Abstract

We have purified to near homogeneity a DNA primase from a mitochondrial fraction of the trypanosomatid Crithidia fasciculata. The enzyme isa single polypeptide chain of 28 kDa. Using a poly(dT) template and ATP as a substrate, the enzyme makes oligonucleotides of which the vastmajority are about 10 nucleotides in size or smaller. With a single-stranded M13 DNA template and the four rNTPs as substrates, the enzyme makes heterogeneous oligonucleotides in the same size range. These oligonucleotides efficiently prime the synthesis of DNA by the Klenow DNApolymerase. Immunolocalization with antibodies against the purified enzyme confirms that the primase is mitochondrial. Furthermore, the enzyme localizes to specific regions of the cell's single mitochondrion, above and below the condensed kinetoplast DNA. The primase does not co-localize with the mitochondrial topoisomerase II and DNA polymerase beta, both of which are associated with two protein complexes positioned on opposite sides of the kinetoplast disc. These localization studies have significant implications for the mechanism of kinetoplast DNA replication.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 01:16:12