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Titolo:
ACV SYNTHETASE - EXPRESSION OF AMINO-ACID ACTIVATING DOMAINS OF THE PENICILLIUM-CHRYSOGENUM ENZYME IN ASPERGILLUS-NIDULANS
Autore:
ETCHEGARAY A; DIECKMANN R; KENNEDY J; TURNER G; VONDOHREN H;
Indirizzi:
TECH UNIV BERLIN,INST BIOCHEM & MOL BIOL,FRANKLINSTR 29 D-10587 BERLIN GERMANY TECH UNIV BERLIN,INST BIOCHEM & MOL BIOL D-10587 BERLIN GERMANY UNIV SHEFFIELD,KREBS INST BIOMOLEC RES,DEPT MOL BIOL & BIOTECHNOL SHEFFIELD S10 2TN S YORKSHIRE ENGLAND UNIV WISCONSIN,SCH PHARM MADISON WI 53706
Titolo Testata:
Biochemical and biophysical research communications
fascicolo: 1, volume: 237, anno: 1997,
pagine: 166 - 169
SICI:
0006-291X(1997)237:1<166:AS-EOA>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
MULTIFUNCTIONAL PEPTIDE SYNTHETASE; DELTA-(L-ALPHA-AMINOADIPYL)-L-CYSTEINYL-D-VALINE SYNTHETASE; STREPTOMYCES-CLAVULIGERUS; CEPHALOSPORIUM-ACREMONIUM; ENNIATIN SYNTHETASE; BIOSYNTHESIS; PROTEINS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
A. Etchegaray et al., "ACV SYNTHETASE - EXPRESSION OF AMINO-ACID ACTIVATING DOMAINS OF THE PENICILLIUM-CHRYSOGENUM ENZYME IN ASPERGILLUS-NIDULANS", Biochemical and biophysical research communications, 237(1), 1997, pp. 166-169

Abstract

Fragments of ACV synthetase of Penicillium chrysogenum carrying partial activities of amino acid activation were expressed under the alcA promoter in an acvA-deletion mutant of Aspergillus nidulans. The 210 kDa domain A-beta-galactosidase fusion protein was partially cleaved to fragments of 200 and 97 kDa. The domain a fragment and the 312 kDa domain BC construct were identified by peptide specific antibodies and shown to catalyze alpha-aminoadipate-, cysteine-, and valine-dependent ATP/[P-32]PPi exchange activity. Substrate specificities were investigated using amino acid analogues. Unexpectedly neither alpha-aminoadipate nor valine activation was exclusive, implying possible misactivations and proof reading functions, Bath fragments were only expressed in limited amounts and found to be unstable. (C) 1997 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 12:24:54