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Titolo:
ENERGY-METABOLISM IN HUMAN-MELANOMA CELLS UNDER HYPOXIC AND ACIDIC CONDITIONS IN-VITRO
Autore:
SKOYUM R; EIDE K; BERG K; ROFSTAD EK;
Indirizzi:
NORWEGIAN RADIUM HOSP,INST CANC RES,DEPT BIOPHYS N-0310 OSLO NORWAY NORWEGIAN RADIUM HOSP,INST CANC RES,DEPT BIOPHYS N-0310 OSLO NORWAY
Titolo Testata:
British Journal of Cancer
fascicolo: 4, volume: 76, anno: 1997,
pagine: 421 - 428
SICI:
0007-0920(1997)76:4<421:EIHCUH>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHINESE-HAMSTER CELLS; MURINE TUMOR-CELLS; TRANSIENT HYPOXIA; INTRACELLULAR PH; SOLID TUMORS; RESISTANCE; GROWTH; OXYGEN; DOXORUBICIN; VIABILITY;
Keywords:
ADENYLATE ENERGY CHARGE; ENERGY METABOLISM; EXTRACELLULAR PH; HYPOXIA; MELANOMA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
R. Skoyum et al., "ENERGY-METABOLISM IN HUMAN-MELANOMA CELLS UNDER HYPOXIC AND ACIDIC CONDITIONS IN-VITRO", British Journal of Cancer, 76(4), 1997, pp. 421-428

Abstract

The response to treatment and the malignant progression of tumours are influenced by the ability of the tumour cells to withstand severe energy deprivation during prolonged exposure to hypoxia at normal or lowextracellular pH (pH(e)). The objective of the present work was to demonstrate intertumour heterogeneity under conditions of microenvironment-induced energy deprivation and to investigate whether the heterogeneity can be attributed to differences in the capacity of the tumour cells to generate energy in an oxygen-deficient microenvironment. Cultures of four human melanoma cell lines (BEX-c, COX-c, SAX-c, WIX-c) wereexposed to hypoxia in vitro at pH(e) 7.4, 7.0 or 6.6 far times up to 31 h by using the steel-chamber method. High-performance liquid chromatography was used to assess adenylate energy charge as a function of exposure time. Cellular rates of glucose uptake and lactate release were determined by using standard enzymatic test kits. The adenylate energy charge decreased with time under hypoxia in all cell lines. The decrease was most pronounced shortly after the treatment had been initiated and then tapered off. BEX-c and SAX-c showed a significantly higheradenylate energy charge under hypoxic conditions than did COX-c and WIX-c whether the pH(e) was 7.4, 7.0 or 6.6, showing that tumours can differ in the ability to avoid energy deprivation during microenvironmental stress. There was no correlation between the adenylate energy charge and the rates of glucose uptake and lactate release. Intertumour heterogeneity in the ability to withstand energy deprivation in an oxygen-deficient microenvironment cannot therefore be attributed mainly todifferences in the capacity of the tumour cells to generate energy byanaerobic metabolism. The data presented here suggest that the heterogeneity is rather caused by differences in the capacity of the tumour cells to reduce the rate of energy consumption when exposed to hypoxia.

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Documento generato il 23/09/20 alle ore 20:23:13