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Titolo:
IMPROVED PCR DETECTION SENSITIVITY OF ERWINIA-CAROTOVORA SUBSP ATROSEPTICA IN POTATO-TUBER PEEL EXTRACT BY PRIOR ENRICHMENT ON A SELECTIVE MEDIUM
Autore:
HYMAN LJ; DEWASMES V; TOTH IK; PEROMBELON MCM;
Indirizzi:
SCOTTISH CROP RES INST DUNDEE DD2 5DA SCOTLAND SCOTTISH CROP RES INST DUNDEE DD2 5DA SCOTLAND
Titolo Testata:
Letters in applied microbiology
fascicolo: 2, volume: 25, anno: 1997,
pagine: 143 - 147
SICI:
0266-8254(1997)25:2<143:IPDSOE>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
SEED;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
13
Recensione:
Indirizzi per estratti:
Citazione:
L.J. Hyman et al., "IMPROVED PCR DETECTION SENSITIVITY OF ERWINIA-CAROTOVORA SUBSP ATROSEPTICA IN POTATO-TUBER PEEL EXTRACT BY PRIOR ENRICHMENT ON A SELECTIVE MEDIUM", Letters in applied microbiology, 25(2), 1997, pp. 143-147

Abstract

A simple and sensitive method was developed to replace the need for complex and laborious DNA extraction to remove inhibitory substances inpotato tuber peel extract before detection of Erwinia carotovora subsp. atroseptica (Eca) by PCR. Eca was enriched by a factor of 10(5) when peel extract was inoculated onto a selective medium, CVP, and incubated at 27 degrees C for 24 h. Bacterial micro-colonies which developedwere suspended in 500 mu l of water and the bacteria diluted in water100-fold, or 10-fold followed by washing by centrifugation, before PCR testing. The sensitivity of detection obtained with the former was ca 10(1)-10(2) cells ml(-1) and with the latter ca 10(1) cells ml(-1), when different numbers of streptomycin-resistant Eca strain were addedto peel extract from three Eca-free potato cultivars. The method was validated and the sensitivity confirmed relative to two different commonly used Eca detection methods using naturally contaminated tubers.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 05:23:21