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Titolo:
MURINE CXCR-4 IS A FUNCTIONAL CORECEPTOR FOR T-CELL-TROPIC AND DUAL-TROPIC STRAINS OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1
Autore:
BIENIASZ PD; FRIDELL RA; ANTHONY K; CULLEN BR;
Indirizzi:
DUKE UNIV,MED CTR,HOWARD HUGHES MED INST,BOX 3025 DURHAM NC 27710 DUKE UNIV,MED CTR,HOWARD HUGHES MED INST DURHAM NC 27710 DUKE UNIV,MED CTR,DEPT GENET DURHAM NC 27710
Titolo Testata:
Journal of virology
fascicolo: 9, volume: 71, anno: 1997,
pagine: 7097 - 7100
SICI:
0022-538X(1997)71:9<7097:MCIAFC>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
INFECTION; RECEPTOR; CLONING; BRAIN; TAT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
P.D. Bieniasz et al., "MURINE CXCR-4 IS A FUNCTIONAL CORECEPTOR FOR T-CELL-TROPIC AND DUAL-TROPIC STRAINS OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1", Journal of virology, 71(9), 1997, pp. 7097-7100

Abstract

The human chemokine receptor hCXCR-4 serves as a coreceptor for T-cell-tropic (T-tropic) and dual-tropic strains of human immunodeficiency virus type I (HIV-1). We have isolated a homolog of hCXCR-4 from a murine T-cell cDNA library and have examined its ability to function as an HIV-1 coreceptor. mCXCR-4 was found to be 91% identical to the humanreceptor at the amino acid level, with sequence differences concentrated in extracellular domains. Surprisingly, coexpression of both hCD4 and mCXCR-4 on either simian or murine cell lines rendered them permissive for HIV-1-indnced cell fusion, indicating that mCXCR-4 is a functional HIV-1 coreceptor. As with hCXCR-4, coreceptor function was restricted to T-tropic and dual-tropic HIV-I strains. Ribonuclease protection analysis indicated that mCXCR-4 mRNA was expressed in only two of six murine cell lines tested. In contrast, Northern blot analysis of human and mouse tissues revealed that CXCR-4 is widely expressed in bothspecies in vivo. Overall, these data suggest that the reported lack of susceptibility of hCD4(+) murine cells to HIV-1 infection in vitro is, at least in part, due to a lack of mCXCR-4 expression rather than alack of coreceptor function.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 17:52:19