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Titolo:
A STREPTOMYCES-GRISEUS GENE (SGAA) SUPPRESSES THE GROWTH DISTURBANCE CAUSED BY HIGH-OSMOLALITY AND A HIGH-CONCENTRATION OF A-FACTOR DURING EARLY GROWTH
Autore:
ANDO N; UEDA K; HORINOUCHI S;
Indirizzi:
UNIV TOKYO,GRAD SCH AGR & LIFE SCI,DEPT BIOTECHNOL,BUNKYO KU TOKYO 113 JAPAN UNIV TOKYO,GRAD SCH AGR & LIFE SCI,DEPT BIOTECHNOL,BUNKYO KU TOKYO 113 JAPAN
Titolo Testata:
Microbiology
, volume: 143, anno: 1997,
parte:, 8
pagine: 2715 - 2723
SICI:
1350-0872(1997)143:<2715:ASG(ST>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
FACTOR-BINDING PROTEIN; SECONDARY METABOLISM; AUTOREGULATORY FACTORS; FACTOR BIOSYNTHESIS; CLONING; DIFFERENTIATION; IDENTIFICATION; DETERMINANT; EXPRESSION; SEQUENCES;
Keywords:
A-FACTOR; AFSA; STREPTOMYCES GRISEUS; A-FACTOR-SENSITIVE STAGE; SGAA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
N. Ando et al., "A STREPTOMYCES-GRISEUS GENE (SGAA) SUPPRESSES THE GROWTH DISTURBANCE CAUSED BY HIGH-OSMOLALITY AND A HIGH-CONCENTRATION OF A-FACTOR DURING EARLY GROWTH", Microbiology, 143, 1997, pp. 2715-2723

Abstract

A-factor socapryloyl-3R-hydroxymethyl-gamma-butyrolactone), produced in a growth-dependent manner, switches on secondary metabolite formation and morphological differentiation in Streptomyces griseus, presumably by binding to the A-factor receptor protein (ArpA)-DNA complex and releasing the repression caused by ArpA. In the A-factor-deficient mutant strain S. griseus HH1 a large deletion includes afsA which is required for A-factor production. Growth and aerial mycelium formation of strain HH1 on media containing high concentrations of sucrose, sorbitol, mannitol, KCI or NaCl was disturbed by the presence of a large amount of A-factor supplied either exogenously or by a high-copy-number plasmid carrying afsA. This disturbance did not occur on media of normalosmolality and was observed only when A-factor was supplied during the very early stage of growth, about 8 h after inoculation. In addition, neither the wild-type strain nor S. griseus KM7 defective in ArpA exhibited the disturbance. These observations suggest that the presence of a large amount of A-factor during the very early stage of growth, probably during the A-factor-sensitive stage, triggered abrupt and disordered expression of some genes. The effect was apparently mediated through ArpA in the A-factor regulatory cascade and disturbed the physiology of strain HH1 under high osmolality. A gene that suppressed the disturbance was identified 5.5 kb upstream of the afsA locus in the wild-type strain. The gene, named sgaA, encoded a protein of 264 aa with a calculated molecular mass of 28 kDa.

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Documento generato il 26/01/21 alle ore 04:48:20