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Titolo:
SUPPRESSION OF ESCHERICHIA-COLI FORMATE HYDROGENLYASE ACTIVITY BY TRIMETHYLAMINE N-OXIDE IS DUE TO DRAINAGE OF THE INDUCER FORMATE
Autore:
ABAIBOU H; GIORDANO G; MANDRANDBERTHELOT MA;
Indirizzi:
INST NATL SCI APPL,CNRS UMR 5577,LAB GENET MOL MICROORGANISMES & INTERACT CELLULAI F-69621 VILLEURBANNE FRANCE INST NATL SCI APPL,CNRS UMR 5577,LAB GENET MOL MICROORGANISMES & INTERACT CELLULAI F-69621 VILLEURBANNE FRANCE CNRS UPR 9043,CHIM BACTERIENNE LAB F-13402 MARSEILLE 20 FRANCE
Titolo Testata:
Microbiology
, volume: 143, anno: 1997,
parte:, 8
pagine: 2657 - 2664
SICI:
1350-0872(1997)143:<2657:SOEFHA>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
DIMETHYL-SULFOXIDE REDUCTASE; 2ND NITRATE REDUCTASE; NUCLEOTIDE-SEQUENCE; GENE-EXPRESSION; NAR OPERON; DEHYDROGENASE; IDENTIFICATION; K-12; SELENOCYSTEINE; PURIFICATION;
Keywords:
ESCHERICHIA COLI K-12; ANAEROBIOSIS; TRIMETHYLAMINE N-OXIDE; FORMATE HYDROGENLYASE; GENE EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
H. Abaibou et al., "SUPPRESSION OF ESCHERICHIA-COLI FORMATE HYDROGENLYASE ACTIVITY BY TRIMETHYLAMINE N-OXIDE IS DUE TO DRAINAGE OF THE INDUCER FORMATE", Microbiology, 143, 1997, pp. 2657-2664

Abstract

The effect of the addition of trimethylamine N-oxide (TMAO) in the growth medium on Escherichia coli anaerobic fermentative and respiratorypathways was examined. Formate dehydrogenase H (FDH-H) activity was totally repressed by the addition of 40 mM TMAO, whereas the overall hydrogenase (HYD) activity was reduced by 25%. Accordingly, expression of lacZ operon fusions with the fdhF and hycB structural genes specifying FDH-H and HYD3 was reduced sevenfold and eightfold, respectively, leading to suppression of an active formate hydrogenlyase system, In contrast, global respiratory formate-dependent phenazine methosulphate reductase (FDH-PMS) activity, which consists of both the major anaerobic FDH-N enzyme and the aerobic FDH-Z isoenzyme, was increased approximately twofold. This was corroborated by a 2.5-fold stimulation of the sole fdoG-uidA transcriptional fusion which reflects the synthesis of the respiratory aerobic FDH-Z enzyme. In fdhD, fdhE or torA mutants lacking either FDH-PMS activity or TMAO reductase (TOR) activity, the formate hydrogenlyase pathway was no longer inhibited by TMAO. In addition, introduction of 30 mM formate in the growth medium was found to relieve the repressive effect of TMAO in the wild-type strain. When TMAOwas added as terminal electron acceptor a significant enhancement of anaerobic growth was observed with the wild-type strain and the fdoG mutant. It was associated with the concomitant suppression of the formate hydrogenlyase enzymes. This was in contrast to the fdnG and torA mutants whose growth pattern and fermentative enzymes remained unaffected. Taken together, these results strongly suggest that formate-dependent reduction of TMAO via FDH-N and TOR reduces the amount of formate available for induction of the formate hydrogenlyase pathway.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 14:21:54