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Titolo:
Detection of infectious enteroviruses and adenoviruses in tap water in urban areas in Korea
Autore:
Lee, SH; Kim, SJ;
Indirizzi:
Seoul Natl Univ, Coll Nat Sci, Sch Biol Sci, Seoul 151742, South Korea Seoul Natl Univ Seoul South Korea 151742 Sci, Seoul 151742, South Korea
Titolo Testata:
WATER RESEARCH
fascicolo: 1, volume: 36, anno: 2002,
pagine: 248 - 256
SICI:
0043-1354(200201)36:1<248:DOIEAA>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; CARCINOMA CELL-LINE; HEPATITIS-A VIRUS; ENTERIC VIRUSES; HUMAN ROTAVIRUS; PCR PROCEDURE; RIVER WATER; WASTE-WATER; NESTED-PCR; TYPE-40;
Keywords:
enterovirus; adenovirus; tap water; cell culture-PCR; sequence analysis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Kim, SJ Seoul Natl Univ, Coll Nat Sci, Sch Biol Sci, Shilim Dong,Kwanak GuSan 56-1, Seoul 151742, South Korea Seoul Natl Univ Shilim Dong,Kwanak Gu San 56-1 Seoul South Korea 151742
Citazione:
S.H. Lee e S.J. Kim, "Detection of infectious enteroviruses and adenoviruses in tap water in urban areas in Korea", WATER RES, 36(1), 2002, pp. 248-256

Abstract

We investigated the viral contamination of tap water at 11 urban sites in Korea between 1997 and 1998 over a period of 11 months. A total of 23 tap water samples were examined for infectious enteroviruses and adenoviruses bya cell culture technique followed by polymerase chain reaction (PCR) amplification. To identify the recovered viruses, sequence analysis of PCR products was performed. Infectious enteroviruses and adenoviruses were detected in 11 (47.8%) and 9 (39.1%) of the samples, respectively. Both enteroviruses and adenoviruses were detected in five samples (21.7%). The level of viral contamination was quite high, ranging from 2 x 10(-3) to 2.9 x 10(-2) Most Probable Number of Infectious Unit L-1, far above the recommended virus level in drinking water set by the US EPA. Poliovirus type 1 derived from vaccine was frequently detected and the remainder comprised coxsackievirus B type or echovirus type 6, which were causative agents of aseptic meningitisin Korea in 1997 and 1998, respectively. Several types of adenovirus were detected in tap water samples and some water samples were found to contain adenoviruses which were closely related to enteric adenovirus types 40 and 41. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 10:35:13