Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Toxicity and uptake mechanism of cylindrospermopsin and lophyrotomin in primary rat hepatocytes
Autore:
Chong, MWK; Wong, BSF; Lam, PKS; Shaw, GR; Seawright, AA;
Indirizzi:
City Univ Hong Kong, Dept Biochem & Chem, Kowloon, Hong Kong, Peoples R China City Univ Hong Kong Kowloon Hong Kong Peoples R China g, Peoples R China Natl Res Ctr Environm Toxicol, Coopers Plains, Qld 4108, Australia Natl Res Ctr Environm Toxicol Coopers Plains Qld Australia 4108 Australia
Titolo Testata:
TOXICON
fascicolo: 2, volume: 40, anno: 2002,
pagine: 205 - 211
SICI:
0041-0101(200202)40:2<205:TAUMOC>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYCLIC PEPTIDE TOXIN; PROTEIN PHOSPHATASES; MICROCYSTIN-LR; RACIBORSKII WOLOSZYNSKA; COLORIMETRIC ASSAY; UMEZAKIA NATANS; HEPATOTOXICITY; INHIBITION; POTENT; MICE;
Keywords:
toxicity; uptake mechanism; cylindrospermopsin; lophyrotomin; primary rat hepatocytes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Lam, PKS City Univ Hong Kong, Dept Biochem & Chem, 83 Tat Chee Ave, Kowloon, Hong Kong, Peoples R China City Univ Hong Kong 83 Tat Chee Ave Kowloon Hong Kong Peoples R China
Citazione:
M.W.K. Chong et al., "Toxicity and uptake mechanism of cylindrospermopsin and lophyrotomin in primary rat hepatocytes", TOXICON, 40(2), 2002, pp. 205-211

Abstract

The toxicities and uptake mechanisms of two hepatotoxins, namely cylindrospermopsin and lophyrotomin, were investigated on primary rat hepatocytes byusing microcystin-LIZ (a well-known hepatotoxin produced by cyanobacteria)as a comparison. Isolated rat hepatocytes were incubated with different concentrations of hepatotoxins for 0, 24, 48 and 72 h. The cell viability wasassayed by the tetrazolium-based (MTT) assay. Microcystin-LR, cylindrospermopsin and lophyrotomin all exhibited toxic effects on the primary rat hepatocytes with 72-h LC50 of 8, 40 and 560 ng/ml, respectively. The involvement of the bile acid transport system in the hepatotoxin-induced toxicities was tested in the presence of two bile acids, cholate and taurocholate. Results showed that the bile acid transport system was responsible for the uptake, and facilitated the subsequent toxicities of lophyrotomin on hepatocytes. This occurred to a much lesser extent with cylindrospermopsin. With its smaller molecular weight, passive diffusion might be one of the possible mechanisms for cylindrospermopsin uptake into hepatocytes. This was supportedby incubating a permanent cell line, KB (devoid of bile acid transport system), with cylindrospermopsin which showed cytotoxic effects. No inhibitionof protein phosphatase 2A by cylindrospermopsin or lophyrotomin was found. This indicated that other toxic mechanisms besides protein phosphatase inhibition were producing the toxicities of cylindrospermopsin and lophyrotomin, and that they were unlikely to be potential tumor promoters. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 09:00:13