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Titolo:
An RGA-like marker detects all known Lr21 leaf rust resistance gene familymembers in Aegilops tauschii and wheat
Autore:
Huang, L; Gill, BS;
Indirizzi:
Kansas State Univ, Throckmorton Plant Sci Ctr, Dept Plant Pathol, Wheat Genet Resources Ctr, Manhattan, KS 66506 USA Kansas State Univ Manhattan KS USA 66506 ces Ctr, Manhattan, KS 66506 USA
Titolo Testata:
THEORETICAL AND APPLIED GENETICS
fascicolo: 6-7, volume: 103, anno: 2001,
pagine: 1007 - 1013
SICI:
0040-5752(200111)103:6-7<1007:ARMDAK>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
DISEASE RESISTANCE; TRITICUM-TAUSCHII; MAP; IDENTIFICATION; CLONING; LINKAGE; CLUSTER; BARLEY; TOMATO; LOCUS;
Keywords:
leaf rust resistance; molecular marker; Aegilops tauschii; sequence-tagged-site;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Gill, BS Kansas State Univ, Throckmorton Plant Sci Ctr, Dept Plant Pathol,Wheat Genet Resources Ctr, Manhattan, KS 66506 USA Kansas State Univ Manhattan KS USA 66506 Manhattan, KS 66506 USA
Citazione:
L. Huang e B.S. Gill, "An RGA-like marker detects all known Lr21 leaf rust resistance gene familymembers in Aegilops tauschii and wheat", THEOR A GEN, 103(6-7), 2001, pp. 1007-1013

Abstract

Leaf rust is one of the most important diseases of wheat worldwide, particularly in the Great Plains region of the USA. One long-term strategy for the control of this disease may be through durable genetic resistance by genepyramiding. An important step in this strategy is identifying molecular markers linked to different leaf rust-resistance genes. Here we report the molecular tagging of a leaf rust-resistance gene that may have the potential for durable resistance through further genetic manipulation and gene pyramiding. Lr39 was previously designated for a leaf rust-resistance gene introgressed from Aegilops tauschii accession TA1675 into the common wheat germplasm WGRC2. Lr40 was designated for a gene derived from Ae. tauschii accession TA1649 and is present in germplasm WGRC7. These genes are now believed to be allelic to Lr21, which was transferred to wheat from a different accession of Ae. tauschii. Molecular mapping of Lr39 and Lr40 indicates that both genes come from TA1649. WGRC2 and WRGC7 also have a similar infection type against rust culture PRTUS6. We suggest the designation of the gene in WGRC2 should be changed to Lr40. RFLP marker KSUD14 (locus Xksud14) was found0.2-cM proximal to Lr40 in a WGRC2/Wichita F-2 population (218 individuals), and co-segregated with the gene in a WGRC7/Wichita F-2 population (165 individuals). A PCR-based molecular marker developed from the sequence-tagged-site (STS) of XksudI4 was mapped to the same locus as the RFLP marker KSUD14 in both populations. KSUD14 has the structure of a resistance gene analog (RGA) including kinase2a and kinase3 domains similar to the Cre3 gene ofwheat and the rust resistance gene Rpl-D of maize. When the PCR products amplified from KSU14 STS were cleaved with restriction enzyme MspI, an 885-bp fragment was found in WGRC2, WGRC7, the Lr21 near-isogenic line, and eight accessions of Ae. tauschii shown to have resistance gene alleles at the Lr21 locus. The KSUD14 PCR-based assay provides an excellent marker for Lr40and Lr21 in diverse wheat breeding and wild Ae. tauschii populations.

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Documento generato il 29/09/20 alle ore 23:47:14