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Titolo:
Molecular mapping of gene Gm-6(t) which confers resistance against four biotypes of Asian rice gall midge in China
Autore:
Katiyar, SK; Tan, Y; Huang, B; Chandel, G; Xu, Y; Zhang, Y; Xie, Z; Bennett, J;
Indirizzi:
Int Rice Res Inst, Div Plant Breeding Genet & Biochem, Manila 1099, Philippines Int Rice Res Inst Manila Philippines 1099 chem, Manila 1099, Philippines Indira Gandhi Agr Univ, Raipur, Madhya Pradesh, India Indira Gandhi Agr Univ Raipur Madhya Pradesh India Madhya Pradesh, India Guangdong Acad Agr Sci, Plant Protect Res Inst, Guangzhou, Peoples R ChinaGuangdong Acad Agr Sci Guangzhou Peoples R China gzhou, Peoples R China
Titolo Testata:
THEORETICAL AND APPLIED GENETICS
fascicolo: 6-7, volume: 103, anno: 2001,
pagine: 953 - 961
SICI:
0040-5752(200111)103:6-7<953:MMOGGW>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
WHEAT-RYE TRANSLOCATION; HESSIAN FLY; DISEASE-RESISTANCE; TRITICUM-TAUSCHII; GM2 GENE; MARKERS; IDENTIFICATION; BACTERIAL; RFLP; SELECTION;
Keywords:
rice (Oryza sativa); gall midge; Orseolia oryzae; RAPD; mapping; sequence tagged sites;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Bennett, J Int Rice Res Inst, Div Plant Breeding Genet & Biochem, POB 933,Manila 1099, Philippines Int Rice Res Inst POB 933 Manila Philippines 1099 Philippines
Citazione:
S.K. Katiyar et al., "Molecular mapping of gene Gm-6(t) which confers resistance against four biotypes of Asian rice gall midge in China", THEOR A GEN, 103(6-7), 2001, pp. 953-961

Abstract

The Chinese rice cultivar Duokang #1 carries a single dominant gene Gm-6(t) that confers resistance to the four biotypes of Asian rice gall midge (Orseolia oryzae Wood-Mason) known in China. Bulked segregant analysis was performed on progeny of a cross between Duokang #1 and the gall midge-susceptible cultivar Feng Yin Zhan using the RAPD method. The RAPD marker OPM06((1400)) amplified a locus linked to Gm-6(t). The locus was subsequently mappedto rice chromosome 4 in a region flanked by cloned RFLP markers RG214 and RG163. Fine mapping of Gm-6(t) revealed that markers RG214 and RG476 flanked the gene at distances of LO and 2.3 cM, respectively. Another gall midge resistance gene, Gm-2, mapped previously to chromosome 4. is located about 16 cM from Gm-6(t). to judge by data from a segregating population derived from a cross between Duokang #1 and the Indian cultivar Phalguna that carries Gm-2. We developed a PCR-based marker-assisted selection kit for transfer of the Gm-6(t) gene into Ming Hui 63 and IR50404, two parental lines commonly used in hybrid rice production in China. The kit contains PCR primer pairs based on the terminal sequences of the RG214 and RG476 clones. Polymorphism between Duokang #1 and the hybrid parental lines was found at these markers after digestion of the PCR products with specific restriction endonucleases. The kit will accelerate introduction of gall midge resistance intohybrid rice in China.

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Documento generato il 20/01/21 alle ore 12:48:01