Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Prostacyclin analogue (OP-2507) induces delayed ex vivo neutrophil apoptosis and attenuates reperfusion-induced hepatic microcirculatory derangement in rats
Autore:
Chen, MF; Chen, JC; Chiu, DF; Ng, CJ; Shyr, MH; Chen, HM;
Indirizzi:
Chang Gung Univ, Coll Med, Chang Gung Mem Hosp, Dept Surg, Taipei, Taiwan Chang Gung Univ Taipei Taiwan Gung Mem Hosp, Dept Surg, Taipei, Taiwan Chang Gung Univ, Coll Med, Chang Gung Mem Hosp, Dept Emergency Med, Taipei, Taiwan Chang Gung Univ Taipei Taiwan Hosp, Dept Emergency Med, Taipei, Taiwan Chang Gung Univ, Coll Med, Chang Gung Mem Hosp, Dept Anaesthesia, Taipei, Taiwan Chang Gung Univ Taipei Taiwan em Hosp, Dept Anaesthesia, Taipei, Taiwan
Titolo Testata:
SHOCK
fascicolo: 6, volume: 16, anno: 2001,
pagine: 473 - 478
SICI:
1073-2322(200112)16:6<473:PA(IDE>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
LEUKOCYTE-ENDOTHELIAL ADHERENCE; ISCHEMIA-REPERFUSION; LIPID-PEROXIDATION; WARM ISCHEMIA; FREE-RADICALS; LIVER; INJURY; TRANSPLANTATION; DYSFUNCTION; CONTRIBUTE;
Keywords:
ischemia-reperfusion; microcirculation; prostacyclin; adherent leukocyte; in vivo microscopy; laser Doppler flowmetry;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Chen, HM Chang Gung Univ, Coll Med, Chang Gung Mem Hosp, Dept Surg, 199 Tung Hwa N Rd, Taipei, Taiwan Chang Gung Univ 199 Tung Hwa N Rd Taipei Taiwan Taipei, Taiwan
Citazione:
M.F. Chen et al., "Prostacyclin analogue (OP-2507) induces delayed ex vivo neutrophil apoptosis and attenuates reperfusion-induced hepatic microcirculatory derangement in rats", SHOCK, 16(6), 2001, pp. 473-478

Abstract

Leukocyte-endothelial adherence and changes of blood flow in microcirculation are associated with the development of ischemia-reperfusion injury in the liver. Polymorphonuclear neutrophil (PMN) apoptosis is essential to maintain homeostasis and plays a major role in limiting the reperfusion-relatedsystemic effects. This study investigates the effects of a prostacyclin analogue (OP-2507) on hepatic ischemia-reperfusion injury. Adult, male Sprague-Dawley rats were used. Five groups were evaluated: (1) sham-operated control, n = 8; (2) ischemia control (1-h ischemia, 5-h reperfusion), n = 8; (3) intravenous infusion with OP-2507 ([15 cis-14-propylcyclohexyl]-16,17,18,19,20-pentanor-9-deoxy-9 alpha ,6-ni-trilo-PGF, methyl eater) at a dose of 1 mug/kg/min plus ischemia, n = 8; (4) intravenous infusion with OP-2507 ata dose of 0.1 mug/kg/min plus ischemia, n = 8, and (5) sham-operated control and intravenous infusion with OP-2507 at a dose of 1 mug/kg/min, N = 8. Laser-Doppler flowmetry and an in vivo microscopy were used to investigate hepatic microcirculation. PMN apoptosis was quantitated by flow-cytometric labeling of DNA strand breaks. Tissue malondialdehyde and adenosine triphosphate were determined at the end of the experiment. Compared with the ischemia: control group, OP-2507 significantly improved harmful insults following ischemia-reperfusion. The changes of mean systemic arterial pressure following ischemia-reperfusion have been significantly attenuated by OP-2507 atboth doses. OP-2507 lessened adherent leukocyte count in the post-sinusoidvenules:, and improved flow velocity in these areas. OP-2507 at both dosesreduced malondialdehyde and increased adenosine triphosphate levels and this effect was dose-related. The activity of delayed ex vivo PMN apoptosis was significantly lower in the ischemia group than that of control and treatment groups. OP-2507 induced the activity of PMN apoptosis and its effect is dose-related, also. The PMN apoptosis activity is strongly correlated with parenchymal damages. This study demonstrates that OP-2507 treatment with ischemia may ameliorate the ischemia-reperfusion injury of the liver in therat model, and increase spontaneous neutrophil apoptosis ex vivo.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 13:14:15