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Titolo:
Three-dimensional visualization and physiologic evaluation of bile canaliculi in the rat liver slice by confocal laser scanning microscopy
Autore:
Yoneyama, K;
Indirizzi:
Showa Univ, Hlth Serv Ctr, Shinagawa Ku, Tokyo 1428666, Japan Showa Univ Tokyo Japan 1428666 v Ctr, Shinagawa Ku, Tokyo 1428666, Japan
Titolo Testata:
SCANNING
fascicolo: 6, volume: 23, anno: 2001,
pagine: 359 - 365
SICI:
0161-0457(200111/12)23:6<359:TVAPEO>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
DYNAMIC ORGAN; CELLS; MICROINJECTION; EXPRESSION; CULTURE; COMMUNICATION; CHOLESTASIS; HEPATOCYTES; BIOLOGY; CA2+;
Keywords:
bile canaliculi; rat liver slice; lucifer yellow; confocal laser scanning microscopy; three-dimensional reconstruction image;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Yoneyama, K Showa Univ, Hlth Serv Ctr, Shinagawa Ku, 1-4-5 Hatanodai, Tokyo 1428666, Japan Showa Univ 1-4-5 Hatanodai Tokyo Japan 1428666 1428666, Japan
Citazione:
K. Yoneyama, "Three-dimensional visualization and physiologic evaluation of bile canaliculi in the rat liver slice by confocal laser scanning microscopy", SCANNING, 23(6), 2001, pp. 359-365

Abstract

We evaluated the morphology and physiologic function of the bile canaliculi (BC) in the rat liver slice (RLS) by confocal laser scanning microscopy (CLSM). Lucifer yellow (LY) dye was injected into the RLS, and the distribution of LY was serially evaluated. After the injection of LY, hepatocytes were initially visualized, followed by visualization of the BC. There was no significant difference in the distribution of LY between zones 1 and 3 in the hepatic lobule. In zone 1, the reticular distribution of the BC was observed, whereas the part of BC was linearly visualized in zone 3 along the course of sinusoids. When changes in the bile canalicular fluorescence (BCF) were serially evaluated, the BCF was decreased to the minimal level (88% ofthe value obtained immediately after the LY injection) 10 min after the LYinjection, and it tended to increase thereafter. The intralobular hepatocyte fluorescence (ILHF) was decreased to 58.9% of the initial value during the first 40 min. However, the ILHF was transiently increased 30 min after the LY injection, suggesting the possibility of reabsorption of LY by hepatocytes. Three-dimensional (3-D) reconstruction images of the BC facilitated the evaluation of the stereoscopic structure of BC. Confocal laser scanningmicroscopy facilitated the evaluation of structures and physiologic function of the BC.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 09:23:03