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Titolo:
Nuclear peroxisome proliferator-activated receptors alpha and gamma have opposing effects on monocyte chemotaxis in endometriosis
Autore:
Hornung, D; Waite, LL; Ricke, EA; Bentzien, F; Wallwiener, D; Taylor, RN;
Indirizzi:
Univ Calif San Francisco, Dept Obstet Gynecol & Reprod Sci, Ctr Reprod Sci, San Francisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA Dept Obstet & Gynecol, D-72076 Tubingen, Germany Dept Obstet & Gynecol Tubingen Germany D-72076 D-72076 Tubingen, Germany
Titolo Testata:
JOURNAL OF CLINICAL ENDOCRINOLOGY AND METABOLISM
fascicolo: 7, volume: 86, anno: 2001,
pagine: 3108 - 3114
SICI:
0021-972X(200107)86:7<3108:NPPRAA>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENDOTHELIAL GROWTH-FACTOR; PERITONEAL-FLUID; PPAR-GAMMA; INFERTILE WOMEN; CYTOKINE RANTES; CELLS; MACROPHAGES; ANGIOGENESIS; APOPTOSIS; RESPONSES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Taylor, RN Univ Calif San Francisco, Dept Obstet Gynecol & Reprod Sci, CtrReprod Sci, HSE 1689, San Francisco, CA 94143 USA Univ Calif San FranciscoHSE 1689 San Francisco CA USA 94143 SA
Citazione:
D. Hornung et al., "Nuclear peroxisome proliferator-activated receptors alpha and gamma have opposing effects on monocyte chemotaxis in endometriosis", J CLIN END, 86(7), 2001, pp. 3108-3114

Abstract

The peroxisome proliferator-activated receptors (PPARs) alpha and gamma are nuclear receptors that play important roles in inflammatory diseases likeulcerative colitis and arthritis. In this study,we examined the possible role of PPARs in macrophage attraction into the peritoneal cavity of patients with endometriosis. We identified PPAR-alpha and gamma messenger RNA by RT-PCR and protein by immunoblotting of lysates of peritoneal macrophages and monocytic:U937 cells. Using immunocytochemistry, we localized PPAR-alpha and -gamma within the nuclei of both cell types. monocyte chemotactic activity of peritoneal fluid from patients with endometriosis was quantified in Boyden chambers. Migration of U937 cells was increased by WY 14643 and reduced by rosiglitazone. Peritoneal fluid from patients with endometriosis activated U937 cells transiently transfected with a PPAR-alpha /GAL4 luciferase reporter. By contrast, peritoneal fluid did not cause significant activation of PPAR-gamma /GAL4 constructs. The U937 cells transiently transfected with a PEAR response element luciferase reporter showed disease stage-dependent up-regulation when treated with peritoneal fluid from patients with endometriosis. Treatment with peritoneal fluid from healthy controls down-regulated PPAR response element transactivation. We conclude that peritoneal fluid of endometriosis patients contains activators of PPAR-alpha that stimulate macrophage chemotaxis. Inhibitors of PPAR-alpha or activators of PPAR-gamma could be developed for the treatment of inflammation associated with endometriosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 07:59:11