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Titolo:
Determination of plasma bromvalerylurea and its main metabolite by a simple high-performance liquid chromatographic method and quantitation of bromide by energy dispersive X-ray spectrometry in carbon tetrachloride-intoxicated rats
Autore:
Tanaka, E; Nakamura, T; Nagashima, A; Yamazaki, K; Ohashi, N; Tsuchihashi, H; Misawa, S;
Indirizzi:
Univ Tsukuba, Inst Community Med, Tsukuba, Ibaraki 3058575, Japan Univ Tsukuba Tsukuba Ibaraki Japan 3058575 sukuba, Ibaraki 3058575, Japan Tsukuba Med Hosp, Tsukuba, Ibaraki 3058558, Japan Tsukuba Med Hosp Tsukuba Ibaraki Japan 3058558 ba, Ibaraki 3058558, Japan Osaka Prefectural Police Headquarters, Osaka 5410053, Japan Osaka Prefectural Police Headquarters Osaka Japan 5410053 5410053, Japan
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 2, volume: 759, anno: 2001,
pagine: 361 - 366
SICI:
1387-2273(20010815)759:2<361:DOPBAI>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLUTATHIONE CONJUGATION; ALPHA-BROMOISOVALERYLUREA; MODEL SUBSTRATE; STEREOSELECTIVITY; PHARMACOKINETICS; EXCRETION; ENANTIOMERS; BILIARY; INVIVO; URINE;
Keywords:
energy-dispersive X-ray spectrometry; bromvalerylurea; carbon tetrachloride;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Tanaka, E Univ Tsukuba, Inst Community Med, Tsukuba, Ibaraki 3058575, Japan Univ Tsukuba Tsukuba Ibaraki Japan 3058575 araki 3058575, Japan
Citazione:
E. Tanaka et al., "Determination of plasma bromvalerylurea and its main metabolite by a simple high-performance liquid chromatographic method and quantitation of bromide by energy dispersive X-ray spectrometry in carbon tetrachloride-intoxicated rats", J CHROMAT B, 759(2), 2001, pp. 361-366

Abstract

In the present study, small volumes of plasma were used for the measurement of bromvalerylurea (BVU), its metabolite, 3-methylbutyrylurea (MVU), and bromide in carbon tetrachloride (CCl4)-treated rats by HPLC-UV and energy dispersive X-ray spectrometry. A liquid-liquid extraction system was also investigated. BVU and MVU were extracted from 100 mul plasma samples in a single-step involving deproteination with 1 M hydrochloric acid using ethenzamide as internal standard. Samples were separated by HPLC in an acetonitrile-8 mM potassium dihydrogenphosphate buffer (35:65, v/v) mobile phase at a flow-rate of 0.4 ml/min on a 15 cm octadecylsilyl column at room temperature. Analytes were detected at a wavelength of 210 nm. The limits of quantitation for BVU, MVU and bromide are 0.1, 0.1 and 50 mug/ml, respectively. The intra-day accuracies over the range of concentrations were 95.8 to 121.1%. 97.2 to 119.7% and 96.2 to 105.8% for BVU, MVU and bromide, respectively. The inter-day accuracies were 97.7 to 115.1%, 98.3 to 111.6% and 98.3 to 102.9% for BVU, MVU and bromide, respectively. The absolute recoveries using tert.-butyl methyl ether are 96-98% for BVU and 95-98% for MVU. The decline in the plasma concentrations of BVU in olive oil-treated rats fitted a one-compartment model and the plasma MVU level reached a peak at around 1.5-2 hand then decreased gradually. The elimination of BVU in CCl4 (1 ml/kg)-treated rats was delayed and MVU production was less than that in the olive oil-treated group. However, there was no difference in the plasma levels of bromide between CCl4-treated rats and control rats. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 20:37:27