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Titolo:
Rapid high-performance liquid chromatographic determination of docetaxel (Taxotere) in plasma using liquid-liquid extraction
Autore:
Ciccolini, J; Catalin, J; Blachon, MF; Durand, A;
Indirizzi:
Fac Pharm Marseille, Lab Toxicocinet & Pharmacocinet, F-13385 Marseille 5,France Fac Pharm Marseille Marseille France 5 cinet, F-13385 Marseille 5,France CHU Timone, Federat Pharmacol Med & Clin & Pharmacocinet, F-13005 Marseille, France CHU Timone Marseille France F-13005 macocinet, F-13005 Marseille, France
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 2, volume: 759, anno: 2001,
pagine: 299 - 306
SICI:
1387-2273(20010815)759:2<299:RHLCDO>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
SOLID-PHASE EXTRACTION; I CLINICAL-TRIAL; PACLITAXEL; PHARMACOKINETICS; METABOLITES; VALIDATION; INFUSION; TAXOIDS;
Keywords:
docetaxel; Taxotere;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Ciccolini, J Fac Pharm Marseille, Lab Toxicocinet & Pharmacocinet, 27 BlvdJean Moulin,F-13385 Marseille 5, France Fac Pharm Marseille 27 Blvd Jean Moulin Marseille France 5 e
Citazione:
J. Ciccolini et al., "Rapid high-performance liquid chromatographic determination of docetaxel (Taxotere) in plasma using liquid-liquid extraction", J CHROMAT B, 759(2), 2001, pp. 299-306

Abstract

A new rapid and sensitive high-performance liquid chromatographic method for analysis of docetaxel (Taxotere) in human plasma was developed and validated. After adding an internal standard (paclitaxel, Taxol), plasma was extracted following a simple liquid-liquid extraction with diethyl ether. Extraction efficiency averaged 95% for docetaxel. Separation was performed using a Nudeosil (C18) 5 mum column, monitored at 227 nm, The isocratic mobile phase consisted of acetonitrile-acetate buffer, pH 5-tetrahydrofuran (45:50:5, v/v) pumped at a flow-rate of 1.8 ml/min. The limit of quantification for docetaxel in plasma was 12.5 ng/ml. Retention times for docetaxel and paclitaxel were 7.7 and 9 min, respectively. Standard curves were linear overa range of 25-1000 ng/ml. This new method is rapid since it does not require time-consuming extraction procedures, or complex chromatographic conditions. This rapidity, along with the lack of chromatographic interferences with various other drugs likely to be administered to the cancer patients (pain killers, corticoids, antiemetics drugs) make this method suitable for daily routine analysis of Taxotere, a major anticancer drug extensively used in clinical oncology. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 03:42:58