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Titolo:
Use of novel solid-phase extraction sorbent materials for high-performanceliquid chromatography quantitation of caffeine metabolism products methylxanthines and methyluric acids in samples of biological origin
Autore:
Georga, KA; Samanidou, VF; Papadoyannis, IN;
Indirizzi:
Aristotelian Univ Salonika, Dept Chem, Analyt Chem Lab, GR-54006 Salonika,Greece Aristotelian Univ Salonika Salonika Greece GR-54006 4006 Salonika,Greece
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 2, volume: 759, anno: 2001,
pagine: 209 - 218
SICI:
1387-2273(20010815)759:2<209:UONSES>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
RP-HPLC ANALYSIS; HUMAN PLASMA; DEMETHYLATED METABOLITES; THEOPHYLLINE; SERUM; DERIVATIVES; SEPARATION; FLUIDS; XANTHINE;
Keywords:
caffeine; methylxanthine; methyluric acid;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Papadoyannis, IN Aristotelian Univ Salonika, Dept Chem, Analyt Chem Lab, GR-54006 Salonika,Greece Aristotelian Univ Salonika Salonika Greece GR-54006 ece
Citazione:
K.A. Georga et al., "Use of novel solid-phase extraction sorbent materials for high-performanceliquid chromatography quantitation of caffeine metabolism products methylxanthines and methyluric acids in samples of biological origin", J CHROMAT B, 759(2), 2001, pp. 209-218

Abstract

An automated reversed-phase high-performance liquid chromatographic (RP-HPLC) method, using a linear gradient elution, is described for the simultaneous analysis of caffeine and metabolites according to their elution order: 7-methyluric acid, 1-methyluric acid, 7-methylxanthine, 3-methylxanthine, l-methylxanthine, 1,3-dimethyluric acid, theobromine, 1,7-dimethyluric acid,paraxanthine and theophylline. The analytical column, an MZ Kromasil C-4, 250x4 mm, 5 mum, was operated at ambient temperature with back pressure values of 80-110 kglcm(2). The mobile phase consisted of an acetate buffer (pH3.5)-methanol (97:3, v/v) changing to 80:20 v/v in 20 min time, delivered at a flow-rate of 1 ml/min. Paracetamol was used as internal standard at a concentration of 6.18 ng/mul. Detection was performed with a variable wavelength UV-visible detector at 275 nm, resulting in detection limits of 0.3 ng per 10-mul injection, while linearity held up to 8 ng/mul for most of analytes, except for paraxanthine and theophylline, for which it was 12 ng/muland for caffeine for which it was 20 ng/mul. The statistical evaluation ofthe method was examined performing intra-day (n=6) and inter-day calibration (n=7) and was found to be satisfactory, with high accuracy and precisionresults. High extraction recoveries from biological matrices: blood serum and urine ranging from 84.6 to 103.0%, were achieved using Nexus SPE cartridges with hydrophilic and lipophilic properties and methanol-acetate buffer(pH 3.5) (50:50, v/v) as eluent, requiring small volumes, 40 mul of blood serum and 100 mul of urine. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 10:18:18