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Titolo:
Depletion of glial cell line-derived neurotrophic factor in substantia nigra neurons of Parkinson's disease brain
Autore:
Chauhan, NB; Siegel, GJ; Lee, JM;
Indirizzi:
Vet Affairs Edward Hines Jr Hosp, Neurol Serv 127, Hines, IL 60141 USA VetAffairs Edward Hines Jr Hosp Hines IL USA 60141 , Hines, IL 60141 USA Vet Affairs Edward Hines Jr Hosp, Res & Dev Serv, Hines, IL 60141 USA Vet Affairs Edward Hines Jr Hosp Hines IL USA 60141 , Hines, IL 60141 USA Loyola Univ, Stritch Sch Med, Dept Neurol, Maywood, IL 60153 USA Loyola Univ Maywood IL USA 60153 Med, Dept Neurol, Maywood, IL 60153 USA Loyola Univ, Stritch Sch Med, Dept Cell Biol Neurobiol & Anat, Maywood, IL60141 USA Loyola Univ Maywood IL USA 60141 l Neurobiol & Anat, Maywood, IL60141 USA Loyola Univ, Stritch Sch Med, Dept Pathol, Maywood, IL 60141 USA Loyola Univ Maywood IL USA 60141 Med, Dept Pathol, Maywood, IL 60141 USA Loyola Univ, Stritch Sch Med, Dept Pharmacol & Expt Therapeut, Maywood, IL60141 USA Loyola Univ Maywood IL USA 60141 l & Expt Therapeut, Maywood, IL60141 USA
Titolo Testata:
JOURNAL OF CHEMICAL NEUROANATOMY
fascicolo: 4, volume: 21, anno: 2001,
pagine: 277 - 288
SICI:
0891-0618(200106)21:4<277:DOGCLN>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-SITU HYBRIDIZATION; DOPAMINE NEURONS; MESSENGER-RNA; TYROSINE-HYDROXYLASE; RETROGRADE TRANSPORT; GROWTH-FACTOR; GENE-THERAPY; FACTOR GDNF; EXPRESSION; RAT;
Keywords:
aging; dopaminergic neurons; image analysis; immunocytochemistry; neuronal loss; neurotrophic factors; Parkinson's disease; substantia nigra;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Siegel, GJ Vet Affairs Edward Hines Jr Hosp, Neurol Serv 127, Hines, IL 60141 USA Vet Affairs Edward Hines Jr Hosp Hines IL USA 60141 60141 USA
Citazione:
N.B. Chauhan et al., "Depletion of glial cell line-derived neurotrophic factor in substantia nigra neurons of Parkinson's disease brain", J CHEM NEUR, 21(4), 2001, pp. 277-288

Abstract

The distribution of nerve growth factor (NGF), ciliary neurotrophic factor(CNTF), glial cell line-derived neurotrophic factor (GDNF), brain derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and neurotrophin-il (NT-4) in substantia nigra pars compacta (SNc) of Parkinson's disease (PD) brains was investigated by immunofluorescence. Cases studied included four 69-77year old neurologically normal male controls and four 72-79 year old male PD patients. Integrated optical densities (IODs) of immunofluorescence overindividual neuromelanin-containing neurons and in areas of neuropil and the number of neurons on H & E stained adjacent sections were quantitated with the use of the BioQuant Image Analyzer. Data were statistically analyzed by ANOVA, including the unpaired two-tailed Student t-test and the Mann-Whitney test. The results showed 55.8% (P < 0.0001) dropout of SNc neurons in PD brains compared to age-matched controls. Despite considerable neuronal dropout, immunofluorescent NTFs in the PD brains showed differential reductions that were consistent within the group as compared to age-matched controls: reductions were GDNF, 19.4%/neuron (P < 0.0001), 20.2%/neuropil (P < 0.0001); CNTF 11.1%/neuron (P < 0.0001), 9.4%/neuropil (P < 0.0001): BDNF, 8.6%/neuron (P < 0.0001), 2.5%/neuropil. NGF, NT-3 and NT-4 showed no significant differences within surviving neurons or neuropil. Since the depletion of GDNF both within surviving neurons and neuropil was twice as great as that of CNTF and BDNF and since the other NTFs showed no changes, GDNF, of the tested NTFs, is probably the most susceptible and the earliest to decrease in the surviving neurons of SNc. These observations suggest a role for decreased availability of GDNF in the process of SNc neurodegeneration in PD. (C) 2001 Elsevier Science B.V. All rights reserved.

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Documento generato il 17/01/20 alle ore 20:53:41