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Titolo:
Control of shape and size of vascular smooth muscle cells in vitro by plasma lithography
Autore:
Goessl, A; Bowen-Pope, DF; Hoffman, AS;
Indirizzi:
Univ Washington, Dept Bioengn, Seattle, WA 98195 USA Univ Washington Seattle WA USA 98195 Dept Bioengn, Seattle, WA 98195 USA Univ Washington, Dept Pathol, Seattle, WA 98195 USA Univ Washington Seattle WA USA 98195 , Dept Pathol, Seattle, WA 98195 USA Univ Washington, Dept Chem Engn, Seattle, WA 98195 USA Univ Washington Seattle WA USA 98195 ept Chem Engn, Seattle, WA 98195 USA
Titolo Testata:
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
fascicolo: 1, volume: 57, anno: 2001,
pagine: 15 - 24
SICI:
0021-9304(200110)57:1<15:COSASO>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
PHENOTYPIC PROPERTIES; GROWTH-CONTROL; SURFACE; LAMININ; ADHESION; DIFFERENTIATION; PROLIFERATION; FIBRONECTIN;
Keywords:
surface-patterning; plasma-lithography; thin film-polymerization; surface analysis; cell size/shape-control; smooth muscle cell culture;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Hoffman, AS Univ Washington, Dept Bioengn, POB 352255, Seattle, WA 98195 USA Univ Washington POB 352255 Seattle WA USA 98195 , WA 98195 USA
Citazione:
A. Goessl et al., "Control of shape and size of vascular smooth muscle cells in vitro by plasma lithography", J BIOMED MR, 57(1), 2001, pp. 15-24

Abstract

The ability to control the shape and size of cells is an important enabling technique for investigating influences of geometrical variables on cell physiology. Herein we present a micropatterning technique ("plasma lithography") that uses photolithography and plasma thin-film polymerization for thefabrication of cell culture substrates with a cell-adhesive pattern on a cell-repellent (non-fouling) background. The micron-level pattern was designed to isolate individual vascular smooth muscle cells (SMC) on areas with aprojected area of between 25 and 3600 mum(2) in order to later study theirresponse to cytokine stimulation in dependence of the cell size and shape as an indication for the phenotypic state of the cells. Polyethylene terephthalate substrates were first coated with a non-fouling plasma polymer of tetraglyme (tetraethylene glycol dimethyl ether). In an organic lift-off process, we then fashioned square- and rectangular-shaped islands of a thin fluorocarbon plasma polymer film of similar to 12-nm thickness. Electron spectroscopy for chemical analysis and secondary ion mass spectroscopy were used to optimize the deposition conditions and characterize the resulting polymers. Secondary ion mass spectroscopy imaging was used to visualize the spatial distribution of the polymer components of the micropatterned surfaces. Rat vascular SMC were seeded onto the patterned substrates in serum-free medium to show that the substrates display the desired properties, and that cell shape can indeed be controlled. For long-term maintenance of these cells, the medium was augmented with 10% calf serum after 24 h in culture, andthe medium was exchanged every 3 days. After 2 weeks, the cells were stillconfined to the areas of the adhesive pattern, and when one or more cells spanned more than one island, they did not attach to the intervening tetraethylene glycol dimethyl ether (tetraglyme) background. Spreading-restrictedcells formed a well-ordered actin skeleton, which was most dense along theperimeter of the cells. The shape of the nucleus was also influenced by the pattern geometry. These properties make the patterned substrates suitablefor investigating if the phenotypic reversion of SMC can be influenced by controlling the shape and size of SMC in vitro. (C) 2001 John Wiley & Sons,Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/21 alle ore 02:19:28