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Titolo:
Quantitative analysis of the hormone-induced hyperacetylation of histone H3 associated with the steroidogenic acute regulatory protein gene promoter
Autore:
Christenson, LK; Stouffer, RL; Strauss, JF;
Indirizzi:
Univ Penn, Ctr Res Reprod & Womens Hlth, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 mens Hlth, Philadelphia, PA 19104 USA Oregon Reg Primate Res Ctr, Div Reprod Sci, Beaverton, OR 97006 USA OregonReg Primate Res Ctr Beaverton OR USA 97006 Beaverton, OR 97006 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 29, volume: 276, anno: 2001,
pagine: 27392 - 27399
SICI:
0021-9258(20010720)276:29<27392:QAOTHH>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
FOLLICLE-STIMULATING-HORMONE; PERI-OVULATORY INTERVAL; MACAQUE GRANULOSA-CELLS; LEYDIG TUMOR-CELLS; BINDING-PROTEIN; STAR GENE; FACTOR-I; TRANSCRIPTIONAL REGULATION; RAPID ACCUMULATION; CORPUS-LUTEUM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Christenson, LK Univ Penn, Ctr Res Reprod & Womens Hlth, 1349 Biomed Res Bldg II-III,421 Curie Blvd, Philadelphia, PA 19104 USA Univ Penn 1349 BiomedRes Bldg II-III,421 Curie Blvd Philadelphia PA USA 19104
Citazione:
L.K. Christenson et al., "Quantitative analysis of the hormone-induced hyperacetylation of histone H3 associated with the steroidogenic acute regulatory protein gene promoter", J BIOL CHEM, 276(29), 2001, pp. 27392-27399

Abstract

Transcriptional regulation of steroidogenic acute regulatory protein (StAR) determines adrenal and gonadal cell steroidogenesis. Chromatin immunoprecipitation assays were combined with quantitative real-time polymerase chainreaction to assess histone acetylation associated with the StAR promoter. MA-10 cells treated with 8-bromo-cAMP had increased acetylated histone H3 associated with the proximal (but not distal) StAR promoter, nascent StAR transcripts, and progesterone production within 15 min, whereas StAR mRNA increased at 30 min. At 360 min, steroidogenesis remained elevated, but mRNA, nascent RNA, and StAR promoter-associated H3 acetylation all declined. StARpromoter-associated H4 acetylation was unchanged by 8-bromo-cAMP treatmentof MA-10 cells. In vivo analysis of macaque and human granulosa cells showed that luteinization was associated with increased StAR promoter-associated H3 acetylation. We conclude that acetylation of H3 (but not H4) associated with the proximal promoter is associated with StAR gene transcription, that chromatin modification occurs in discrete regions of the promoter, that the initial steroidogenic response to 8-bromo-cAMP occurs prior to increased StAR mRNA accumulation, and that MA-10 cell StAR gene transcription and promoter-associated H3 acetylation are biphasic during a 6-h treatment period. The union of the chromatin immunoprecipitation assay with quantitative real-time polymerase chain reaction described and validated here should enhance the analysis of gene expression.

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Documento generato il 27/09/20 alle ore 22:32:46