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Titolo:
Mechanical stimulation induces pp125(FAK) and pp60(src) activity in an in vivo model of trabecular bone formation
Autore:
Moalli, MR; Wang, SQ; Caldwell, NJ; Patil, PV; Maynard, CR;
Indirizzi:
Univ Michigan, Dept Orthopaed Surg, Orthopaed Res Labs, Ann Arbor, MI 48109 USA Univ Michigan Ann Arbor MI USA 48109 ed Res Labs, Ann Arbor, MI 48109 USA
Titolo Testata:
JOURNAL OF APPLIED PHYSIOLOGY
fascicolo: 2, volume: 91, anno: 2001,
pagine: 912 - 918
SICI:
8750-7587(200108)91:2<912:MSIPAP>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
FOCAL ADHESION KINASE; SIGNAL-TRANSDUCTION; EXTRACELLULAR-MATRIX; ENDOTHELIAL-CELLS; SHEAR-STRESS; EXPRESSION; INTEGRIN; MECHANOTRANSDUCTION; CYTOSKELETON; HEPATOCYTES;
Keywords:
mechanotransduction; tyrosine phosphorylation; animal model;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Moalli, MR 400 N Ingalls,Rm G161, Ann Arbor, MI 48109 USA 400 N Ingalls,Rm G161 Ann Arbor MI USA 48109 bor, MI 48109 USA
Citazione:
M.R. Moalli et al., "Mechanical stimulation induces pp125(FAK) and pp60(src) activity in an in vivo model of trabecular bone formation", J APP PHYSL, 91(2), 2001, pp. 912-918

Abstract

Utilizing an in vivo model of trabecular bone formation, we demonstrated the temporal and spatial activation of pp125(FAK) in response to specific mechanical load stimuli. Bone chambers equipped with hydraulic actuators wereaseptically inserted into each proximal tibial metaphysis of adult, male dogs under general anesthesia. The load stimulus consisted of a trapezoidal waveform, with a maximum compressive load of 17.8 N, loading rate of 89 N/s, at 1 Hz frequency. One chamber was loaded for 2 (120 cycles), 15 (900 cycles), or 30 min (1,800 cycles), whereas the contralateral chamber served asunloaded control. Bone chambers were biopsied at postload time points of 0, 15, and 45 min. Load-induced activation of FAK was rapid, and the duration of activation was dependent on the number of applied load cycles. Mechanical stimulation increased the association of FAK with Src and the time course of complex formation paralleled the temporal activation of FAK. Evaluation of cryosections revealed prominent FAK immunoreactivity among marrow fibroblasts and stromal cells.

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Documento generato il 31/03/20 alle ore 09:11:45